摘要
为了发展能够同时兼顾超分辨、快速成像和视场的荧光显微镜,以促进其在活细胞或微观动态过程成像的应用,将压缩感知应用到超分辨荧光显微镜中,利用投影梯度稀疏重构算法对单帧荧光宽场图像重构,并进行了理论分析、仿真和实验验证。结果表明,该方法能够突破光学衍射极限,成像分辨率达到180nm,相比衍射极限提高1.8倍。此结果说明压缩感知能够实现单帧宽场超分辨荧光显微成像,相比现有的方法在成像速度上有巨大的提升。
In order to develop fluorescent microscope that can simultaneously take into account super-resolution,fast imaging and field of view,and promote its application in imaging of living cells or micro-dynamic processes,the compressed sensing was applied to super-resolution fluorescence microscopy.The algorithm of gradient projection for sparse reconstruction was used to reconstruct single fluorescence wide field image.Theoretical analysis,simulation and experimental verification were carried out.The results show that,this method can break through the optical diffraction limit.The imaging resolution is 180nm.Compared with the diffraction limit,it is 1.8 times higher.Compressed sensing can realize single-frame wide-field super-resolution fluorescence microscopy imaging.Compared with the existing methods,the imaging speed has been greatly improved.
作者
李文文
刘书朋
王中阳
LI Wenwen;LIU Shupeng;WANG Zhongyang(Key Laboratory of Specialty Fiber Optics and Optical Access Networks,Shanghai Institute for Advanced Communication&Data Science,Shanghai University,Shanghai 200444,China;Shanghai Advanced Research Institute,Chinese Academy of Sciences,Shanghai 201210,China)
出处
《激光技术》
CAS
CSCD
北大核心
2020年第2期196-201,共6页
Laser Technology
基金
国家重点研发计划资助项目(2016YFC0100603)。
关键词
显微
超分辨
压缩感知
投影梯度稀疏重构算法
microscopy
super-resolution
compressed sensing
gradient projection for sparse reconstruction
