摘要
目的探讨喉癌组织中微小核糖核酸(microRNA,miR)-34a和Survivin表达与临床病理特征和预后的关系。方法选取2010年5月至2013年12月手术治疗的96例喉癌患者为研究对象,分别取手术切除的喉癌组织及癌旁正常黏膜组织。采用RT-PCR检测miR-34a和Survivin mRNA表达,免疫组织化学法检测Survivin蛋白表达,分析miR-34a和Survivin表达与患者临床病理特征的关系。Spearman等级相关分析喉癌组织miR-34a和Survivin表达的相关性,Kaplan-Meier法分析miR-34a和Survivin表达对喉癌患者生存率的影响,Cox比例风险回归模型分析影响喉癌患者的预后因素。结果与癌旁正常黏膜组织相比,喉癌组织中miR-34a表达水平显著降低(P<0. 01),Survivin mRNA表达水平显著升高(P<0. 01)。Survivin主要表达于细胞质。与癌旁正常黏膜组织相比,喉癌组织Survivin蛋白阳性表达率显著升高(67. 71%vs 7. 29%,P<0. 01)。喉癌组织中miR-34a和Survivin mRNA高表达、低表达在TNM分期、肿瘤分化程度以及淋巴结是否转移方面有统计学差异(P<0. 05,P<0. 01)。Spearman相关性分析显示,喉癌组织中miR-34a和Survivin表达呈负相关(r=-0. 461,P<0. 05)。miR-34a高表达患者5年生存率高于低表达患者(80. 65%vs 63. 08%,P<0. 05);Survivin高表达患者5年生存率低于低表达患者(62. 32%vs85. 19%,P<0. 05)。Cox回归模型分析结果显示,miR-34a和Survivin均是影响喉癌患者预后的独立因素(P<0. 01)。结论喉癌组织中miR-34a表达下调,Survivin表达上调,两者呈明显负相关,可能是喉癌诊治的新靶点和预后判断标记物。
Objective To investigate the correlation between microRNA(miR)-34a and Survivin expression and clinicopathological features and prognosis in laryngeal cancer tissues. Methods A total of 96 patients with laryngeal cancer from May 2010 to December 2013 were selected as research subjects,and resceted laryngeal cancer tissue and normal mucosa tissue adjacent to the cancer were used as the research objects. Real-time reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of miR-34a and Survivin mRNA,and immunohistochemical method was used to detect the expression of Survivin protein. Spearman rank correlation analysis was used to analyze the correlation between miR-34a and Survivin expression in laryngeal cancer tissue,Kaplan-Meier method was used to analyze the effect of miR-34a and Survivin expression on the survival of laryngeal cancer patients,and Cox proportional hazard regression model was used to analyze the prognostic factors of laryngeal cancer patients. Results Compared with the normal mucosa tissue adjacent to the cancer,the expression level of miR-34a in laryngeal cancer tissue was significantly reduced(P< 0. 01),and Survivin mRNA expression level was significantly increased(P< 0. 01). Survivin is mainly expressed in the cytoplasm.Compared with normal mucosa tissue adjacent to the cancer,the positive expression rate of Survivin protein in laryngeal cancer tissue was significantly increased(67. 71% vs 7. 29%,P< 0. 01). The high expression and low expression of miR-34a and Survivin mRNA in laryngeal cancer tissues were statistically different in TNM stage,tumor differentiation and whether lymph nodes metastasized(P< 0. 05,P< 0. 01). Spearman correlation analysis showed that miR-34a expression were negatively correlated with Survivin expression in laryngeal cancer tissues(r =-0. 461,P< 0. 05). The 5-year survival of patients with high expression of miR-34a was higher than that of patients with low expression(80. 65% vs63. 08%,P< 0. 05);while the 5-year survival of patients with high expression of Survivin was lower than that of patients with low expression(62. 32% vs 85. 19%,P< 0. 05). Cox regression model analysis showed that miR-34a and Survivin were independent factors affecting the prognosis of patients with laryngeal cancer(P< 0. 01). Conclusions The expression of miR-34a is down-regulated and the expression of Survivin is up-regulated in laryngeal cancer tissues,and there was a significant negative correlation between them,which may be new targets and prognostic markers for diagnosis and treatment of laryngeal cancer.
作者
要兆旭
韩海平
冯志星
YAO Zhao-xu;HAN Hai-ping;FENG Zhi-xing(Department of Otolaryngology and Head Surgery,Handan Central Hospital,Handan,Hebei 056000,China)
出处
《中国临床研究》
CAS
2020年第2期145-149,共5页
Chinese Journal of Clinical Research
基金
河北省医学科学研究重点课题计划项目(20181667)~~
