摘要
为研究喷施外源褪黑素对烟草幼苗不同程度干旱胁迫条件下的影响效应。以烟草幼苗为试验材料,探究喷施100μmol·L^-1外源褪黑素对干旱胁迫下幼苗的生长、光合特性、抗氧化酶活性以及渗透调节物质含量的影响。结果表明,干旱严重影响植物生长,喷施褪黑素缓解了干旱胁迫造成的植株生长抑制,并通过提高叶片叶绿素含量和净光合速率(Pn)、气孔导度(Gs)、蒸腾速率(Tr),降低胞间CO2浓度(Ci),同时增强超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性,减少O-2和丙二醛(MDA)的含量,显著增加渗透调节物质的积累,以提高烟草幼苗的抗干旱胁迫能力,结果为褪黑素对烟草幼苗的抗旱性影响机理提供依据。
In order to study the effects of exogenous melatonin on tobacco seedlings under different drought stress levels,the growth,photosynthetic characteristics,antioxidant enzymes activities and osmotic regulatory substances contents of tobacco seedlings under drought stress were investigated after sprayed 100μmol·L^-1 melatonin.The results showed that plant growth was inhibited by drought stress;spraying melatonin alleviated the growth of tobacco plants inhibited by drought stress.Melatonin spraying improved the chlorophyll content and net photosynthetic rate(Pn),stomatal conductance(Gs),transpiration rate(Tr)of tobacco plant leaves,decreased the intercellular CO2 concentration(Ci).Moreover,melatonin application enhanced the activities of superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),reduced O-2 and malondialdehyde(MDA)content,and increased the accumulation of osmotic regulation substances significantly.Therefore melatonin application can improve drought stress resistance of tobacco seedling.The present research provided reference for studying the mechanism of effects of melatonin on drought resistance in tobacco seedlings.
作者
贺嘉豪
陈建中
徐坚强
向金友
杨懿德
张学伟
宁尚辉
王林
刘园
杨洋
景延秋
程玉渊
HE Jiahao;CHEN Jianzhong;XU Jianqiang;XIANG Jinyou;YANG Yide;ZHANG Xuewei;NING Shanghui;WANG Lin;LIU Yuan;YANG Yang;JING Yanqiu;CHENG Yuyuan(College of Tobacco,Henan Agricultural University,Zhengzhou 450002,China;Nanyang Cigarette Factory,Henan China Tobacco Industry Co.,Ltd.,Henan Nanyang 473000,China;Changde City Company of Hunan Tobacco Company,Hunan Changde 415000,China;Yibin City Company of Sichuan Tobacco Company,Sichuan Yibin 644002,China;Guangdong China Tobacco Industry Co.,Ltd.,Guangzhou 510310,China;China Tobacco Hubei Industrial LLC,Wuhan 430051,China;Nanyang Company of Henan Tobacco Company,Henan Nanyang 473000,China)
出处
《中国农业科技导报》
CAS
CSCD
北大核心
2020年第2期50-57,共8页
Journal of Agricultural Science and Technology
基金
南阳市公司重大专项(2018411301240127)
湖南省烟草公司常德市公司技术开发研究项目(2018430700240157)
四川省烟草公司宜宾市公司科技攻关项目(201851150024089)
广东中烟工业有限责任公司项目(2019440000340016)
中国烟草总公司四川省烟草公司项目(2019410102000453)
湖北中烟工业有限责任公司项目(2018420000340277)
关键词
褪黑素
烟草幼苗
干旱胁迫
生理机制
melatonin
tobacco seedlings
drought stress
physiological mechanism
