摘要
为研究重组白介素2(Recombinant interleukin-2,r IL-2)对红鳍东方鲀(Takifugu rubripes)血液生化指标及免疫指标的影响,选用体重为(205.9±30.5)g的红鳍东方鲀进行了重组白介素2的免疫应答实验。设计r IL-2免疫剂量为2.5、5、7.5、10μg,并在免疫后的4 h、8 h和12 h进行采血。结果表明:经r IL-2免疫后红鳍东方鲀血清中尿素氮含量在各个浓度组的8 h时间点显著高于同组内其它时间点(P<0.05),4 h时间点甘油三酯含量有随着免疫浓度增加而增加的趋势,谷草转氨酶活性在8 h时间点要显著高于其他时间点(P<0.05),2.5μg、5μg浓度组过氧化氢酶的活性在4 h时间点显著高于其他各组(P<0.05),对免疫球蛋白的含量也有显著影响。本研究发现,r IL-2的免疫可引起血液生化指标和免疫指标的变化,尤其是超氧化物歧化酶和谷草转氨酶的变化较为显著(P<0.05),可在一定程度上促进红鳍东方鲀的免疫反应。
In order to study the effects of recombinant interleukin-2(r IL-2)on blood biochemical parameters and immune indexes of Takifugu rubripes,the T.rubripes with a body weight of(205.9±30.5)g was selected.These T.rubripes were used for the immune response assay of recombinant interleukin 2.The r IL-2 immunization dose was designed to be 2.5,5,7.5,and 10μg,and blood was collected at 4 h,8 h,and 12 h after immunization.The results showed that the content of urea nitrogen in T.rubripes serum after r IL-2 immunization was significantly higher than that in other groups at the 8h time point(P<0.05),and the triglyceride content at 4 h time point tended to increase with the rise of immunization dosage.The activity of aspartate aminotransferase at 8 h was higher than that at other time points(P<0.05).The activity of catalase in 2.5 and 5μg dosage group was significantly higher than that of other groups at 4 h(P<0.05).It also had a significant effect on the content of immunoglobulin.This study found that the injection of recombinant IL-2 caused changes in blood biochemical indicators and immune indexes,especially the changes of superoxide dismutase and aspartate aminotransferase(P<0.05),which may promote the immune response of T.rubripes to a certain extent.
作者
保明秀
藏林
张红斌
刘福君
荆笛
王秀利
BAO Ming-xiu;ZANG Lin;ZHANG Hong-bin;LIU Fu-jun;JING Di;WANG Xiu-li(College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023)
出处
《生物技术通报》
CAS
CSCD
北大核心
2020年第3期177-182,共6页
Biotechnology Bulletin
基金
国家海水鱼产业技术体系岗位科学家项目(CARS-47)
国家重点研发计划重点专项(2018YFD0900301-10)
