摘要
[目的]观察活血温通方对缺氧诱导H9c2心肌细胞损伤的保护作用。[方法]采用H9c2心肌细胞进行缺氧无血清刺激,同时加入活血温通方含药血清和空白血清处理4 h后,检测细胞活力、Caspase 3和Caspase 8活性、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、丙二醛(MDA)含量,并进行二氢乙啶(DHE)染色观察活性氧(ROS)变化,以及Hoechst染色观察细胞凋亡情况,对比分析各组之间差异。[结果]与对照组比较,缺氧组H9c2心肌细胞活力、SOD和CAT活性显著下降(P<0.01),Caspase 3和Caspase 8活性、MDA含量明显升高(P<0.01),同时累积的ROS含量和细胞凋亡数量增多(P<0.01)。活血温通方含药血清能提升细胞活力、增加SOD、CAT活性(P<0.01),明显降低MDA水平、Caspase 3和Caspase 8活性(P<0.01),同时减少ROS累积,抑制心肌细胞凋亡(P<0.01)。[结论]通过抑制细胞凋亡、减轻氧化应激,活血温通方一定程度上可以保护心肌细胞免受缺氧损伤。
[Objective] To observe the protective effect of Huoxue Wentong Formula on hypoxia-induced injury of H9c2 myocardial cells.[Methods] H9c2 myocardial cells were stimulated by hypoxia and serum-free,and treated with serum containing Huoxue Wentong Formula and blank serum for 4 hours. Cell viability,Caspase 3 and Caspase 8 activities,SOD and CAT activities,MDA content were measured. The changes of ROS were observed by DHE staining,and the apoptotic cells was detected by Hoechst staining. The significant differences among the groups were compared. [Results] Compared with the control group,hypoxic group H9c2 myocardial cell viability,the activities of SOD and CAT decreased significantly(P<0.01),Caspase 3 and Caspase 8 activities,MDA content increased significantly(P<0.01),while the cumulative ROS content and the number of cell apoptosis were increased markedly(P<0.01). However,the serum containing Huoxue Wentong Formula could increase notablely cell viability,SOD and CAT activities(P <0.01),reduce MDA level,Caspase 3 and Caspase 8 activities significantly(P<0.01),and alleviate ROS accumulation,inhibit cardiomyocyte apoptosis(P<0.01).[Conclusion] Huoxue Wentong Formula protected myocardial cells from hypoxia injury to a certain extent by inhibiting apoptosis and alleviating oxidative stress.
作者
刘甜甜
王擎擎
姚魁武
刘友明
段锦龙
LIU Tiantian;WANG Qingqing;YAO Kuiwu;LIU Youming;DUAN Jinlong(Guang’anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)
出处
《天津中医药》
CAS
2020年第3期322-325,共4页
Tianjin Journal of Traditional Chinese Medicine
基金
国家自然科学基金面上项目（81873173）
关键词
活血温通方
缺氧
心肌细胞
活性氧
凋亡
Huoxue Wentong Formula
hypoxia
cardiomyocyte
reactive oxygen species
apoptosis
