摘要
目的探讨组蛋白赖氨酸N-甲基转移酶(EZH2)和肌动蛋白结合蛋白(Fascin)在侵袭性垂体腺瘤组织中的表达水平及与其临床预后的关系。方法回顾性收集2013年5月至2016年5月榆林市第一医院收治的102例垂体腺瘤患者的临床资料,将其分为侵袭组(n=50)和非侵袭组(n=52),采用免疫组化法检测两组患者垂体腺瘤中EZH2、Fascin的阳性表达率;Pearson相关系数分析EZH2、Fascin的阳性表达率的相关性;采用单因素和多因素分析影响侵袭性垂体腺瘤患者预后的相关因素;利用Kaplan-Meier法分析EZH2、Fascin蛋白表达与侵袭性垂体腺瘤患者3年生存率的关系。结果侵袭组患者EZH2、Fascin阳性率分别为74.00%、66.00%,明显高于非侵袭组的51.92%、30.77%,差异均有统计学意义(P<0.05);Pearson相关性分析显示:EZH2与Fascin阳性表达水平呈正相关(r=0.672,P<0.001);单因素及多因素分析结果显示:肿瘤≥3 cm、EZH2阳性及Fascin阳性水平是影响垂体腺瘤预后的独立危险因素(P<0.05);侵袭组患者中EZH2、Fascin阴性表达者的生存率分别为92.31%(12/13)、88.24%(15/17),明显高于阳性者的54.05%(20/37)、51.52%(17/33),差异均有统计学意义(P<0.05)。结论EZH2、Fascin蛋白阳性表达可作为判断侵袭性垂体腺瘤发生、发展的重要指标,且对侵袭性垂体腺瘤患者具有较好的预后价值。
Objective To explore the expression levels of enhancer of zeste homolog 2(EZH2)and actin binding protein(Fascin)in invasive pituitary adenomas(IPA)tissue and their relationship with clinical prognosis.Methods The clinical data of 102 patients with pituitary adenoma who were admitted to Yulin First Hospital during the period from May 2013 to May 2016 were retrospectively collected.They were divided into invasion group(n=50)and non-invasion group(n=52).The positive expression rates of EZH2 and Fascin in pituitary adenomas in both groups were detected by immunohistochemistry.Pearson correlation coefficients were applied to analyze the correlation between positive expression rates of EZH2 and Fascin.Univariate and multivariate analysis were applied to analyze related factors affecting prognosis of IPA patients.Kaplan-Meier method was applied to analyze the relationship between expression of EZH2,Fascin,and 3-year survival rate in IPA patients.Results The positive rates of EZH2 and Fascin in invasion group were 74.00%,66.00%,significantly higher than 51.92%,30.77%in non-invasion group(P<0.05).Pearson correlation analysis showed that positive expression levels of EZH2 and Fascin were positively correlated(r=0.672,P<0.001).The results of univariate and multivariate analysis showed that tumors not shorter than 3 cm,positive levels of EZH2 and Fascin were independent risk factors affecting prognosis of IPA patients(P<0.05).The survival rates in IPA patients with negative expression of EZH2 and Fascin were 92.31%(12/13),88.24%(15/17),significantly higher than54.05%(20/37),51.52(17/33)in IPA patients with positive expression of EZH2 and Fascin(P<0.05).Conclusion The positive expressions of EZH2 and Fascin can be applied as important indexes to judge occurrence and development of IPA,which are of good prognostic value for IPA patients.
作者
周峰
何华
ZHOU Feng;HE Hua(Department of Neurosurgery,Yulin First Hospital,Yulin 718000,Shaanxi,CHINA;Department of Ophthalmology,Yulin First Hospital,Yulin 718000,Shaanxi,CHINA)
出处
《海南医学》
CAS
2020年第5期586-589,共4页
Hainan Medical Journal
