摘要
建立了离子交换色谱—柱后衍生法测定绿茶饮料中39种游离氨基酸的分析方法。样品经过样品稀释剂稀释,经过磺酸型阳离子交换色谱柱分离,茚三酮柱后衍生,通过调节流动相比例和柱温温度,达到最好的分离效果。结果表明39种游离氨基酸均得到了较好的分离,在摩尔浓度1~100nmol/L范围内线性良好,相关系数(r)均大于0.99,其中肌氨酸、β-氨基丙酸、β-氨基异丁酸、肌肽、鹅肌肽、乙醇胺、磷-乙醇胺等7种游离氨基酸的检出限为10μg/L,定量限为30μg/L,其余32种游离氨基酸的检出限为1μg/L,定量限为3μg/L;加标回收率在74.0%~101.7%,RSD%(n=6)在0.30%~4.8%,满足检测要求。方法前处理简单易操作,实用性强,适用于绿茶饮料中39种游离氨基酸的检测。
An analytical method was developed for the determination of 39 free amino acids in green tea beverages by ion exchange chromatography-postcolumn derivatization.The sample was diluted by the sample diluent,separated by a sulfonic acid cation exchange chromatography column,and used ninhydrin for Post-column derivatization.The best separation effect was achieved by adjusting the flow ratio and column temperature.The results showed that 39 kinds of free amino acids were well separated,and the linearity was good in the range of molar concentration from 1 to 100 nmol/L,and the correlation coefficients(r)were all greater than 0.99.Among them,the detection limit of 7 free amino acids such as sarcosine,β-aminopropionic acid,β-amino isobutyric acid,carnosine,goose carnosine,ethanolamine,and phosphorus-ethanolamine is 10μg/L,the limit of quantification is 30μg/L,and the detection limit of the remaining 32 free amino acids is 1μg/L.The limit is 3μg/L;the spiked recovery is between 74.0%and 101.7%,and the RSD%(n=6)is between 0.3%and 4.8%,which meets the testing requirements.The pretreatment method is simple,easy to operate,and highly practical.It is suitable for the detection of 39 free amino acids in green tea beverages.
作者
贺习文
高勤叶
黄姣姣
李易轩
张兴悟
马川
HE Xi-wen;GAO Qin-ye;HUANG Jiao-jiao;LI Yi-xuan;ZHANG Xing-wu;MA Chuan(Shaanxi QinYun Agricultural Products Inspection and Testing Co.,Ltd,Weinan 714000,China)
出处
《饮料工业》
2020年第1期35-40,共6页
Beverage Industry
关键词
绿茶
饮料
离子交换色谱
柱后衍生
游离氨基酸
green tea
beverage
ion exchange chromatography
post-column derivatization
free amino acids
