摘要
[目的]检测鼻咽癌组织中干扰素调节因子-5(IRF-5)的表达,探讨其在鼻咽癌细胞CNE-1增殖、迁移与侵袭中的作用。[方法]采用定量聚合酶链反应(qPCR)测定鼻咽癌旁组织与鼻咽癌组织中IRF-5的表达水平。随机将CNE-1细胞分为正常对照组、空白病毒组和病毒转染组,分别采用MTT法测定各组细胞的增殖情况,采用划痕实验测定各组细胞的迁移能力,采用Transwell小室法测定各组细胞的侵袭能力,采用qPCR和Western blot法测定各组细胞IRF-5、金属蛋白酶-2(MMP-2)、E-cadherin、趋化因子-12(CXCL-12)和趋化因子受体-4(CXCR-4)mRNA和蛋白的表达水平。[结果](1)鼻咽癌组织中IRF-5 mRNA水平较癌旁组织显著降低(P<0.05);(2)病毒转染组细胞在培养24h、48h和72h时OD值均较正常对照组和空白病毒对照组显著降低(P<0.05);(3)病毒转染组细胞的划痕宽度较正常对照组和空白病毒对照组显著增大(P<0.05);(4)病毒转染组穿膜细胞数量较正常对照组和空白病毒对照组显著降低(P<0.05);(5)病毒转染组细胞的IRF-5和E-cadherin mRNA表达水平较正常对照组和空白病毒对照组显著升高,而MMP-2、CXCL-12和CXCR-4 mRNA表达水平较正常对照组和空白病毒对照组显著降低(P<0.05)。[结论] IRF-5的低表达可能与鼻咽癌的发生发展有关,过表达IRF-5能够抑制鼻咽癌细胞的增殖、迁移和侵袭,IRF-5可能通过增加E-cadherin表达而抑制MMP-2、CXCL-12和CXCR-4表达而发挥作用。
[Objective] To investigate the expression of interferon regulatory factor-5(IRF-5) in nasopharyngeal carcinoma(NPC) tissues,and its roles in the proliferation,migration and invasion of NPC cells.[Methods] The expression level of IRF-5 was measured by quantitative polymerase chain reaction(qRTPCR) in 65 specimens of NPC tissue and corresponding pericancerous mucosa. Human nasopharyngeal carcinoma CNE-1 cells were randomly divided into normal control group,blank virus group and virus transfection group,and the proliferation of cells were determined by MTT method,the migration ability of cells were determined by scratch test,the invasive ability of cells were determined by Transwell assay,and mRNA and protein expression levels of IRF-5,metalloproteinase-2(MMP-2),e-cadherin,chemokine-12(CXCL-12) and chemokine receptor-4(Cxcr-4) were determined by qRT-PCR and Western blot,respectively. [Results] The expression level of IRF-5 mRNA in NPC tissues was significantly lower than that in pericancerous tissues(P<0.05). OD values of cells in the virus transfection group were significantly lower than those in the normal control group and the blank virus group at 24 h,48 h and 72 h after culture(P<0.05). The scratch width of cells in the viral transfection group was significantly larger than that of the normal control group and the blank virus group(P<0.05). The number of membrane penetrating cells in the virus transfection group was significantly lower than that in the normal control group and the blank virus group(P<0.05). The expression levels of IRF-5 and E-cadherin mRNA in cells of the virus transfection group were significantly higher than those of the normal control group and the blank virus group,while expression levels of MMP-2,CXCL-12 and CXCR-4 mRNA were significantly lower than those of the normal control group and the blank virus group(P<0.05).[Conclusion] The low expression of IRF-5 may be related to the occurrence and development of NPC. Overexpression of IRF-5 could inhibit the proliferation,migration and invasion of NPC cells,and IRF-5 may inhibit the expression of MMP-2,CXCL-12 and CXCR-4 by increasing the expression of E-cadherin.
作者
高飞
贾霖
韩建军
王允
贾利
陈宓
何君
刘小军
代红春
GAO Fei;JIA Lin;HAN Jian-jun;WANG Yun;JIA Li;CHEN Mi;HE Jun;LIU Xiao-jun;DAI Hong-chun(The Third Hospital of Mianyang,Sichuan Mental Health Center,Mianyang 621000,China)
出处
《肿瘤学杂志》
CAS
2019年第12期1064-1069,共6页
Journal of Chinese Oncology
基金
四川省卫计委2018科研课题（18PJ210)
关键词
鼻咽肿瘤
干扰素调节因子-5
细胞迁移
细胞侵袭
,nasopharyngeal carcinoma
interferon regulatory factor-5
cell migration
cell invasion
