摘要
目的:比较不同来源生脉注射液对新西兰兔和猫的降压作用,探讨新西兰兔用于生脉注射液降压作用研究和产品质量评价的可行性。方法:取健康新西兰兔16只和猫4只,均雌雄各半,分别经耳缘静脉缓慢注射50mg·kg^-1(新西兰兔)或腹腔注射45mg·kg^-1(猫)的戊巴比妥钠进行麻醉,按1.0mL·kg^-1的给药体积分别给予A、B和C三个厂家生脉注射液后,立即测定其动脉血压。统计分析比较新西兰兔和猫对不同来源生脉注射液的降压反应。结果:新西兰兔对1.0mL·kg^-1的不同厂家生脉注射液均具有很好的降压反应,降压均值大于3kPa,且不同来源产品对新西兰兔的降压作用存在一定的差异性,在16只兔体内试验结果均为一致;而猫对生脉注射液的降压反应具有敏感性较高但易耐受的特点。结论:新西兰兔不仅可以用于生脉注射液降压作用的研究,还可用于产品间质量的评价。
Objective:To compare the depressor effects of Shengmai injections produced by different manufacturers on New Zealand rabbits and cats,and explore the quality of Shengmai injections in New Zealand rabbits.Methods:Sixteen healthy New Zealand rabbits and 4 cats were anesthetized with pentobarbital sodium by injecting slowly into the ear vein of New Zealand rabbits at the dose of 50 mg·kg^-1 or intraperitoneally at the dose of45 mg·kg^-1 for cats,respectively.Then,all of animals were injected intravenously with Shengmai injections from A,B,and C manufacturers respectively at dose of 1.0 m L·kg^-1 BW.Arterial blood pressure was immediately measured with an acquisition system for physiological signal measurements,and a statistical analysis was carried out to compare the depressor response of New Zealand rabbits and cats to Shengmai injections from different manufacturers.Results:New Zealand rabbits had good depressor response to 1.0 m L·kg^-1 Shengmai injections,with more than 3 k Pa decrease mean value of blood pressure.Besides,the depressor effects of different Shengmai injections on New Zealand rabbits were different,and the difference was consistent in sixteen testing rabbits.However,the depressor response of cats to Shengmai injections was highly sensitive but easily tolerated.Conclusion:New Zealand rabbit can be used to study the depressor effects of Shengmai injection,and evaluate the quality differences among products.
作者
黎家敏
马佳丽
杨春
邓林
马文静
荣祖元
Li Jia-min;Ma Jia-li;Yang Chun;Deng Lin;Ma Wen-jing;Rong Zu-yuan(Sichuan Institute for Food and Drug Control,Chengdu 611731,Sichuan)
出处
《四川生理科学杂志》
2020年第1期1-5,10,共6页
Sichuan Journal of Physiological Sciences
基金
四川省食品药品检验检测院院立项目（编号：2018-KYYL-007)
关键词
生脉注射液
新西兰兔
猫
降压作用
Shengmai injections
Laboratory rabbits
Cats
Depressor effect
