摘要
以17005和19501这2个胡萝卜品种为试验材料,研究种子灭菌条件以及基因型、外植体、培养基和激素对胡萝卜愈伤组织诱导的影响。结果表明,2个品种在无水乙醇灭菌30 s后再用20%H2O2溶液灭菌20 min,出苗率最高;2个品种均以下胚轴为外植体诱导的愈伤组织出愈率较高,其中,17005品种诱导率达到100%,19501品种诱导率也都在90%以上;在MS和B5培养基中同时添加2,4-D和6-BA诱导愈伤组织的效果要比只添加2,4-D的好,但相同激素条件下的MS和B5培养基诱导效果差异不显著。
Two carrot varieties,17005 and 19501,were used as materials to study seed sterilization conditions and the effects of genotype,explant,medium and hormone on carrot callus induction in this experiment.The results showed that the emergence rate of these two cultivars was the highest when they were sterilized with 20%H2O2 solution for 20 min after sterilization in absolute ethanol for 30 s.Both varieties got the highest callus introduction using hypocotyls as explants.The induction rate of 17005 variety was 100%,and that of 19501 variety was also over 90%.The effect of callus introduction of adding 2,4-D and 6-BA together to MS and B5 medium was better than only adding 2,4-D,but under the same hormone conditions,the induction effect with MS and B5 medium was not significantly different.
作者
刘真真
常双峰
冯羽飞
王晓东
武喆
LIU Zhenzhen;CHANG Shuangfeng;FENG Yufei;WANG Xiaodong;WU Zhe(College of Horticulture,Shanxi Agricultural University,Taigu 030801,China;Linfen Agricultural Environmental Monitoring Station,Linfen 041000,China)
出处
《山西农业科学》
2020年第3期294-297,共4页
Journal of Shanxi Agricultural Sciences
基金
国家自然科学基金项目(31601751)
山西省重点研发计划重点项目(201703D211001-04-01)
山西农业大学科技创新基金项目(2016ZZ02)。
