摘要
为了解龙眼BRI1基因家族的生物学功能及响应光照机制,对其BRI1基因成员鉴定、基因结构、蛋白保守结构域、启动子顺式作用元件、互作miRNA预测、不同体胚发生阶段和不同组织器官的FPKM值及其响应光照表达模式等进行分析.结果表明:DlBRI1基因家族包含4个成员,分别命名为DlBRI1-1、DlBRI1-2a、DlBRI1-2b和DlBRI1-3.DlBRI1是一种无内含子基因,无内含子基因在转录的过程中不需要经历内含子的剪切步骤,是响应外界因素的一种快速应答基因.龙眼BRI1蛋白家族为植物富亮氨酸重复类受体蛋白激酶的一种,其在植物激素信号转导和非生物胁迫中具有重要调控作用.龙眼BRI1四个家族成员启动子均含有大量的光响应元件、激素应答元件、非生物胁迫响应元件,表明龙眼BRI1家族基因可能是连接光信号转导与激素信号转导的重要纽带.DlBRI1-3为miR390e的靶基因.FPKM值分析表明,DlBRI1-1和DlBRI1-3在体胚发生过程和不同组织部位中均呈现高表达,推测DlBRI1-1和DlBRI1-3可能在龙眼整个生长发育过程中起到更为关键的作用.荧光定量PCR结果推测,蓝光信号使得miR390的表达量显著减少,导致靶基因BRI1-3的表达量增加,从而影响油菜素内脂从属基因BZR1、转录因子PIF4,进而影响龙眼功能性代谢产物积累.本研究表明DlBRI1具有功能多样性,可能在龙眼响应光信号、激素信号、非生物胁迫及代谢调控中发挥作用.
By investigating the biological function and responsive lighting mechanism of the BRI1 gene family in Dimocarpus longan Lour, the DlBRI1 gene family members were identified and their gene structure, protein domain, cis-acting element of promoter, mutual miRNA, FPKM value of somatic embryogenesis at different stages and different tissues in longan and response light expression mode were analyzed. The DlBRI1 gene family contains four members, named DlBRI1-1, DlBRI1-2a, DlBRI1-2b, and DlBRI1-3, respectively. DlBRI1 is an intronless gene that does not require an intron cleavage step during transcription and responds quickly to external factors. Longan BRI1 protein family is a plant leucine-rich-repeat receptor-like kinase that plays an important regulatory role in plant hormone signal transduction and abiotic stress. Four family members of longan BRI1 promoters contains a large number of light-responsive elements, hormone-responsive elements, and abiotic stress responsive elements, indicating that the longan BRI1 family genes may be linked to light signal transduction and hormonal signal transduction. DlBRI1-3 is a target gene of miR390e. The FPKM value analysis indicated that DlBRI1-1 and DlBRI1-3 had high expression in somatic embryogenesis and different tissues. It is speculated that DlBRI1-1 and DlBRI1-3 might play a more critical role in the whole growth and development of longan. Fluorescence quantitative PCR results speculated that the blue light signal resulted in significant reduction in miR390e expression that can cause an increase in the expression of target gene BRI1-3, thereby affecting the brassinosteroid-dependent gene BZR1 and transcription factor PIF4. Finally, this affected the accumulation of functional metabolites in longan. This study indicates that DlBRI1 has functional diversity and might play an important role in longan response to light signals, hormonal signals, abiotic stresses, and metabolism.
作者
李汉生
孙刚
陈晓慧
姚德恒
林玉玲
赖钟雄
LI Hansheng;SUN Gang;CHEN Xiaohui;YAO Deheng;LIN Yuling;LAI Zhongxiong(College of Resources and Chemical Engineering,Sanming University,Sanming 365004,China;Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2020年第1期125-134,共10页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(31572088、31672127)
福建省高原学科建设经费(102/71201801101)
福建农林大学科技创新专项基金(CXZX2018076、CXZX2017189、CXZX2017314、CXZX2016118、KF2015108)
三明学院引进高层次人才科研启动经费(19YG06,18YG01)资助。
关键词
龙眼
BRI1基因家族
成员鉴定
光照
miRNA预测
表达
Dimocarpus longan Lour.
BRI1 family
gene identification
light
miRNA prediction
expression analysis
