摘要
目的探讨血清中免疫球蛋白以及血清蛋白电泳检测在继发性肾病综合征患者中的临床应用价值。方法选择吉林大学第二医院2015年1月至2018年12月收治的继发性肾病综合征患者154例,经临床诊断分为狼疮性肾炎组49例、糖尿病肾病组52例、过敏性紫癜性肾炎组31例、多发性骨髓瘤性肾炎组22例,同时选取50例健康体检者作对照。用琼脂糖凝胶电泳法对所有样本进行血清蛋白电泳检测,透射比浊法检测血清中免疫球蛋白IgG、IgA、IgM含量。结果血清蛋白电泳结果显示,四组患者血清白蛋白与正常对照组相比均有降低(P<0.05),以多发性骨髓瘤性肾炎组降低最为明显;四组患者血清α1球蛋白、α2球蛋白均有不同程度的升高(P<0.05),以狼疮性肾炎组增高最为明显;狼疮性肾炎、糖尿病肾病、过敏性紫癜性肾炎的β球蛋白显著高于正常对照(P<0.05),而多发性骨髓瘤性肾炎增高不明显;狼疮性肾炎、过敏性紫癜性肾炎、多发性骨髓瘤性肾炎γ球蛋白均高于正常对照(P<0.05),尤其以多发性骨髓瘤性肾炎增高明显,而糖尿病肾病的γ球蛋白与对照组相比略有降低,但无统计学差异。免疫球蛋白测定结果显示,狼疮性肾炎、过敏性紫癜性肾炎、多发性骨髓瘤性肾炎IgG高于正常对照(P<0.05),而以多发性骨髓瘤性肾炎升高最为显著;四组患者IgM均高于正常对照(P<0.05)。结论肾脏疾病多伴有机体蛋白质代谢紊乱,不同类型肾脏疾病血清蛋白电泳结果主要取决于原发疾病,通过血清蛋白电泳联合免疫球蛋白检测对继发性肾病综合征的诊断有重要的临床意义。
Objective To investigate the clinical application value of serum immunoglobulin and serum protein electrophoresis in patients with secondary nephrotic syndrome.Methods A total of 154 patients with secondary nephrotic syndrome admitted to the Second Hospital of Jilin University from January 2015 to November 2018 were enrolled.The clinical diagnosis was divided into 49 cases of lupus nephritis group,52 cases of diabetic nephropathy group,31 cases of the allergic purpura nephritis group and 22 cases in the multiple myeloma nephritis group.50 healthy patients were selected as controls.All samples were subjected to serum protein electrophoresis detection by agarose gel electrophoresis,and the content of immunoglobulin IgG,IgA and IgM in serum was detected by turbidimetry.Results Serum protein electrophoresis showed that the serum albumin of the four groups was lower than that of the normal control group(P<0.05),and the decrease was most obvious in the multiple myeloma nephritis group.The serumα1 globulin andα2 sphere were increased in different degrees in four groups(P<0.05),and the increase was most obvious in the lupus nephritis group.Theβ-globulin of lupus nephritis,diabetic nephropathy and allergic purpuric nephritis was significantly higher than the normal control(P<0.05),the increase in multiple myeloma nephritis was not obvious;γglobulin in lupus nephritis,allergic purpuric nephritis,multiple myeloma nephritis gamma globulin were higher than normal control(P<0.05),especially in multiple myeloma nephritis group,theγglobulin in diabetic nephropathy was slightly lower than that of the control group,but there was no statistical difference.The results of immunoglobulin assay showed that IgG in lupus nephritis,allergic purpuric nephritis and multiple myeloma nephritis was higher than that in normal controls(P<0.05),but the most common increase in multiple myeloma nephritis;four groups of patients IgM was higher than the normal control(P<0.05).Conclusion Kidney disease is associated with disorder of organism protein metabolism.The serum protein electrophoresis results of different types of kidney diseases mainly depend on the primary disease.The detection of secondary nephrotic syndrome by serum protein electrophoresis combined with immunoglobulin detection has important clinical significance.
作者
刘秀敏
武婧
邓沫
朱洪权
LIU Xiu-min;WU Jin;DENG Mo(Department of Clinical Laboratory,Second Hospital of Jilin University,Changchun Jilin 130041,China)
出处
《中国实验诊断学》
2020年第2期246-249,共4页
Chinese Journal of Laboratory Diagnosis
关键词
血清蛋白电泳
免疫球蛋白
肾病综合征
Serum protein electrophoresis
Immunoglobulin
Nephrotic syndrome
