摘要
目的:优化超声波辅助提取7种地黄成方制剂中含量测定工艺。方法:以马钱苷和丹皮酚为考察指标,通过单因素试验考察超声波功率、超声波频率、提取容器、提取溶剂、提取时间、料液比、提取温度,采用L16(45)正交试验设计确定最佳提取工艺,对市场不同厂家成方制剂进行测定。结果:超声波功率在250~600 W,超声波频率在25~60 KHz提取效果比较,差异无统计学意义(P>0.05);采用玻璃容器提取,影响超声波辅助提取的主次因素为提取时间>料液比>提取溶剂>提取温度,最佳提取工艺提取时间为30 min、料液比为1∶100、提取溶剂为45%甲醇、提取温度为50℃。除六味地黄软胶囊外,其他6种超声波辅助提取均优于传统加热提取。结论:超声波辅助提取能显著缩短提取时间、简化实验操作,可用于马钱苷和丹皮酚的含量测定。
Objective:To optimize the ultrasonic-assisted extraction process for the determination of seven preparations of Radix Rehmanniae.Methods:Taking loganin and paeonol as indexes,the ultrasonic power,frequency,extraction container,extraction solvent,extraction time,Solid-liquid ratio and extraction temperature were investigated by single factor experiment.The optimum extraction process was determined by L16(45)orthogonal design.And the preparations from different manufacturers were determined.Results:There was no significant difference in the extraction effect(P>0.05)with the ultrasonic power of 250-600 W and the ultrasonic frequency of 25-60 KHz.Extraction by glass container,the effectiveness of factors on extraction was in this order:extraction time,Solid-liquid ratio,extraction solvent,extraction temperature.The optimized extraction conditions are as follows:extraction time with 30 min,Solid-liquid ratio 1∶100,45%methanol,extraction temperature with 50℃.Ultrasonic-assisted extraction were superior to traditional heating extraction except Liuwei Dihuang Soft Capsule(六味地黄软胶囊).Conclusion:Ultrasound-assisted extraction can significantly shorten the extraction time and simplify the experimental operation.It can be used for the determination of loganin and paeonol.
作者
潘小红
赵薇
刘玉玲
殷帅
龙凌云
林海
PAN Xiao-hong;ZHAO Wei;LIU Yu-ling;YIN Shuai;LONG Ling-yun;LIN Hai(Hunan Institute for Pharmaceutical Inspection,Changsha Hunan 410001,China)
出处
《中医药导报》
2020年第3期1-5,共5页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
湖南省自然科学基金项目(2016JJ6079)。
关键词
超声波辅助提取
丹皮酚
马钱苷
正交试验
工艺优化
ultrasound-assisted extraction
paeonol
loganin
orthogonal test
process optimization
