摘要
以1月生杉木幼苗为材料,设置对照(CK)和铝胁迫(0.5 mmol·L-1)处理,研究铝胁迫对杉木幼苗生长、叶绿素和类胡萝卜素质量分数、叶绿素荧光特性、光合气体交换参数及叶绿体超微结构的影响。结果表明:①铝胁迫下杉木幼苗根系伸长、生物量显著受抑,铝胁迫处理还显著降低杉木叶片叶绿素和类胡萝卜素质量分数;②铝胁迫下杉木叶片净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(Tr)均显著下降,而胞间二氧化碳摩尔分数(Ci)则显著升高;③铝胁迫下杉木叶片PSⅡ最大光化学效率(Fv/Fm)、PSⅡ潜在活性(Fo/Fv)、光化学淬灭系数(Qp)均显著下降,而非光化学淬灭系数(Np,q)则显著增加;④透射电镜结果显示,铝胁迫使杉木叶片叶绿体膨大隆起,叶绿体内基质片层解体,基粒片层数量显著减少,且分布不均,排列松散。可见,铝介导对杉木叶片叶绿体内部结构的破坏,是导致铝胁迫下杉木叶片叶绿素和类胡萝卜素质量分数降低及光合能力下降的主要原因,从而最终抑制杉木幼苗的生长。
One-month-old Cunninghamia lanceolata seedlings were employed to investigate the effects of aluminum(Al)stress on the growth of seedlings,content of chlorophyll and carotenoid,chlorophyll fluorescence characteristics,photosynthetic gas exchange parameters,and chloroplast ultrastructure in leaves of C.lanceolata under both control(CK)and Al(0.5 mmol·L-1)treatments.The relative root elongation and seedling biomass of C.lanceolata were significantly inhibited,moreover,the contents of chlorophyll and carotenoid were also significantly decreased under Al stress.The net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)in leaves of C.lanceolata seedlings were greatly decreased under Al stress,while the opposite was true for intercellular CO2 content(Ci).Al treatment significantly decreased the PSⅡmaximal photochemical efficiency(Fv/Fm),PSⅡpotential efficiency(Fo/Fv)and photochemical quenching coefficient(Qp),while the significantly increased of non-photochemical quenching coefficient(Np,q)was observed in leaves of C.lanceolata seedlings.By transmission electron microscope,under Al stress,the chloroplast in leaves of C.alanceolata seedlings was swelling bulge,the stroma lamellas were destroyed,the numbers of grana lamellas were significantly decreased,and were loosely and unevenly distributed in the chloroplast.Taken together,the above results demonstrated that Al-induced growth inhibition in C.lanceolata seedlings mainly through destroying the ultrastructure of chloroplast,which resulted in a significantly decreased of contents of chlorophyll and carotenoid,these in turn greatly suppressed the photosynthetic capacity of C.lanceolata seedlings,and ultimately the growth of C.lanceolata was inhibited.
作者
叶义全
洪凯
张家君
曹光球
林思祖
杨强
许珊珊
Ye Yiquan;Hong Kai;Zhang Jiajun;Cao Guangqiu;Yang Qiang;Xu Shanshan(Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China,State Forestry and Grassland Administration Engineering Research Center of Chinese Fir)
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2020年第2期7-11,16,共6页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(31700535)
福建省自然科学基金项目(2017J01608)
福建省高校青年自然基金重点项目(JZ160433)。
关键词
杉木
铝胁迫
超微结构
叶绿体
光合特性
Cunninghamia lanceolata
Aluminum stress
Ultrastructure
Chloroplast
Photosynthetic characteristic
