摘要
目的:了解EBV感染与肾病及相关疾病的关系。方法:回顾性研究方法。收集2018年7月-2019年6月我院儿童肾内科435例住院患儿的血液样本,其中肾病综合征患儿101例,急性肾小球肾炎患儿102例和过敏性紫癜患儿232例,采用实时荧光定量PCR方法检测其外周血单个核细胞中EBV-DNA,并对检测结果进行统计学分析。结果:肾病及相关疾病患儿EBV总阳性率和病毒载量明显高于对照组(χ^2=24.946,P=0.000;Z=-2.725,P=0.006),而男女患儿之间阳性率和病毒载量均差异无统计学意义;不同肾病及相关疾病间EBV阳性率明显不同(χ^2=21.523,P=0.000),急性肾小球肾炎EBV的阳性率最高,高达71.57%,过敏性紫癜次之,为51.72%,肾病综合征最低,为39.60%,但不同疾病之间EBV阳性者的病毒载量无明显差异。结论:非典型性原发EBV感染在肾相关疾病中普遍存在,在预防和治疗儿童肾病及相关疾病时,将EBV的感染作为其中的因素之一进行考虑,诊断会更准确、预后会更好。
Objective:To understand the relationship between EBV infection and nephropathy and related diseases.Methods:A retrospective study was conducted in 435 cases of nephropathy disease and related diseases in our hospital from July 2018 to June 2019,including 101 children with nephrotic syndrome,102 children with acute glomerulonephritis and 232 cases of allergic purpura.The viral load of EBV in PBMC of children was detected by real-time fluorescence quantitative PCR,and the results were statistically analyzed.Results:The total positive rate and viral load of EBV in children with kidney disease and related diseases were significantly higher than those in control group(χ^2=24.946,P=0.000;Z=-2.725,P=0.006),but there was no significant difference in positive rate and viral load between male and female children.The positive rate of EBV was significantly different among different kidney diseases and related diseases(χ^2=21.523,P=0.000).The positive rate of EBV in acute glomerulonephritis was the highest(71.57%),that of Henoch-Schonlein purpura was 51.72%,and that of nephrotic syndrome was the lowest(39.60%).However,there was no significant difference in the load of EBV among different diseases.Conclusion:Because of the prevalence of atypical primary EBV infection,the infection of EBV should be considered as one of the factors in the prevention and treatment of kidney disease and related diseases in children.So that the diagnosis will be more accurate and the prognosis will be better.
作者
江杨华
宋然
李艳
林应标
JIANG Yanghua;SONG Ran;LI Yan(The First People’s Hospital of Chenzhou,Chenzhou City,Hu’nan Province 423000)
出处
《医学理论与实践》
2020年第6期881-882,888,共3页
The Journal of Medical Theory and Practice
基金
郴州市第一人民医院科学研究项目(N2019-039)。
