摘要
【目的】明确马铃薯Y病毒(potato virus Y,PVY)侵染后诱导的烟草营养成分的变化及其对烟蚜Myzus persicae生命特性的影响,旨在进一步解析PVY-烟草-烟蚜三者间的互作机制。【方法】通过蒽酮比色法和氨基酸自动分析仪测定了PVY不同侵染时期烟株体内的可溶性糖和游离氨基酸含量的变化;测定和比较了感病与健康烟草植株上烟蚜种群生长发育、成虫寿命、繁殖力和有翅蚜产生量的差异性。【结果】PVY侵染前、中、后期(分别为侵染后5,12和20 d)的烟草叶片中游离氨基酸的总量均显著高于健康烟草叶片。相较于健康烟草叶片,在PVY侵染前期的烟草叶片中,谷氨酸、脯氨酸、天冬氨酸、色氨酸、缬氨酸、赖氨酸和组氨酸的含量显著增加;PVY侵染中期,感病叶片中丝氨酸含量显著下降,谷氨酸、天冬氨酸、色氨酸、缬氨酸、亮氨酸、苯丙氨酸、精氨酸和组氨酸含量显著提高;PVY侵染后期,感病叶片中甘氨酸含量显著下降,谷氨酸、脯氨酸、天冬氨酸、苏氨酸、缬氨酸、亮氨酸、丙氨酸、苯丙氨酸、组氨酸、酪氨酸和精氨酸含量显著提高。在PVY侵染的前期和中期,感病叶片中的可溶性糖含量显著高于健康烟叶,而在侵染后期感病叶片中可溶性糖含量显著低于健康烟草叶片的。PVY侵染前期和中期的烟草叶片中总糖和总游离氨基酸的含量比值显著高于健康烟草叶片中的。在PVY侵染的烟草植株和健康烟草植株上取食的烟蚜其发育历期、若蚜历期、成蚜繁殖期、繁殖后期、寿命、烟蚜种群的内禀增长率、周限增长率和平均世代周期均无显著差异,但在感病烟草植株上取食的烟蚜成蚜繁殖前期显著缩短,其繁殖力和净生殖率显著提高。相较于健康烟草植株,在PVY侵染烟草植株上定殖的烟蚜种群有翅蚜发生的高峰期提前。【结论】PVY侵染前期和中期提高了寄主烟草的营养品质,从而提高了烟蚜的繁殖力。侵染后期烟草营养品质的下降,促使烟蚜种群有翅蚜的产生和扩散,从而有利于PVY自身的传播。
【Aim】This study aims to evaluate the changes in nutrients in tobacco induced by potato virus Y(PVY)and their effects on the life characteristics of the green peach aphid,Myzus persicae,so as to clarify the interaction mechanism between PVY-tobacco and aphids.【Methods】Changes in the soluble sugar and free amino acid contents in tobacco plants at different stages of PVY infection were determined using the anthrone colorimetric method and an automatic amino acid analyzer,respectively.The differences in population development parameters,adult longevity,fecundity,and the number of alataes of M.persicae on PVY-infected tobacco plants and healthy tobacco plants were investigated and compared.【Results】The free amino acid contents in tobacco leaves at the early,steady,and late stages of PVY infection(5,12 and 20 d post infection,respectively)were significantly higher than those in the healthy leaves.Moreover,the contents of glutamic acid,proline,aspartic acid,tryptophan,valine,lysine,and histidine in tobacco leaves at the early stage of PVY infection were also significantly higher than those in the healthy plants.Moreover,at the steady stage of PVY infection,the serine content in tobacco leaves was significantly decreased,whereas the contents of glutamic acid,aspartic acid,tryptophan,valine,leucine,phenylalanine,arginine,and histidine were significantly increased as compared with those in the healthy leaves.At the late stage of PVY infection,the glycine content in tobacco leaves was significantly decreased,whereas the contents of glutamic acid,proline,aspartic acid,threonine,valine,leucine,alanine,phenylalanine,histidine,tyrosine,and arginine were significantly increased as compared with those in the healthy leaves.Furthermore,the soluble sugar contents in tobacco leaves at the early and steady stages of PVY infection were significantly higher than that in the healthy leaves,but significantly lower at the late stage of infection.The content ratios of total sugars to total free amino acids in tobacco leaves at the early and steady stages of PVY infection were significantly higher than that in the healthy leaves.Analysis of life table data showed that there were no significant differences in the developmental duration,pre-adult duration,reproductive period,post-reproductive period,longevity,intrinsic rate of increase,finite rate of increase and mean generation time of M.persicae on the PVY-infected and healthy plants.However,as compared with the aphids on the healthy plants,the aphids on the PVY-infected plants had a significantly shorter pre-reproductive period,and significantly higher fecundity and net reproductive rate.The M.persicae population on the PVY-infected plants showed an advanced peak of alatae emergence relative to that on the healthy plants.【Conclusion】The nutritional quality of tobacco host is improved at the early and steady stages of PVY infection,thus improving the fecundity of M.persicae.However,the decline in the nutritional quality of tobacco plants at the late stage of infection might contribute to the earlier emergence and spread of the alataes,which is conducive to PVY transmission.
作者
陈茜
刘金燕
徐蓬军
刘英杰
董勇浩
臧云
蔡宪杰
任广伟
CHEN Xi;LIU Jin-Yan;XU Peng-Jun;LIU Ying-Jie;DONG Yong-Hao;ZANG Yun;CAI Xian-Jie;REN Guang-Wei(Key Laboratory of Tobacco Integrated Pest Management,Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao,Shandong 266101,China;Shanghai Tobacco Group Co.,Ltd.,Shanghai 200082,China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2020年第2期181-190,共10页
Acta Entomologica Sinica
基金
山东省现代农业产业技术体系烟草产业创新团队(SDAIT-25-03)
中国烟草总公司重点实验室项目(110201603011)
上海烟草集团有限责任公司项目(K2018-1-010P)。
关键词
烟蚜
马铃薯Y病毒
烟草
营养代谢
生命特性
Myzus persicae
Potato virus Y
tobacco
nutritional metabolism
life characteristics
