摘要
目的研究丙泊酚对高糖所致大鼠原代成骨细胞氧化应激损伤的影响。方法采用SD仔鼠颅骨片组织培养法获取原代成骨细胞,分为高糖组、丙泊酚组和对照组。高糖组培养液为α-MEM培养基+10%胎牛血清+25.5 mmol/L葡萄糖,丙泊酚组培养液为α-MEM培养基+10%胎牛血清+25.5 mmol/L葡萄糖+30 mg/ml丙泊酚,对照组培养液为α-MEM培养基+10%胎牛血清。取培养后的3组原代成骨细胞,利用CCK-8法测定细胞增殖活力,流式细胞仪检测细胞凋亡率,Western blot检测细胞Caspase-3蛋白表达水平,并试剂盒检测细胞活性氧(ROS)表达水平。结果培养第3天,对照组和丙泊酚组原代成骨细胞增殖活力明显优于高糖组(P<0.05)。与对照组比较,高糖组原代成骨细胞凋亡率、Caspase-3蛋白以及ROS荧光强度水平明显升高(P<0.05)。与高糖组比较,丙泊酚组原代成骨细胞凋亡率、Caspase-3蛋白以及ROS荧光强度水平明显降低(P<0.05)。结论高糖可以导致大鼠原代成骨细胞氧化应激损伤,增加细胞凋亡;丙泊酚可以减轻高糖所致大鼠原代成骨细胞的氧化应激,减少细胞凋亡,促进细胞增殖,为治疗高血糖状态下的骨骼系统疾病提供了新的途径。
Objective To study the effect of propofol on oxidative stress injury induced by high glucose in primary rat osteoblasts.Methods Primary osteoblasts were obtained by tissue culture of newborn SD rat skull slices and divided into high glucose group,propofol group and control group.The culture solution of high glucose group,propofol group and control group includedα-MEM medium containing 10%fetal bovine serum and 25.5 mmol/L glucose,α-MEM medium containing 10%fetal bovine serum,25.5 mmol/L glucose and 30 mg/ml propofol,andα-MEM medium containing 10%fetal bovine serum,respectively.Three groups of primary osteoblasts were cultured.CCK-8 method was used to determine the cell proliferation activity,and flow cytometry was used to detect the apoptosis rate.Western blot was used to detect the expression level of Caspase-3 protein,and the kit was used to detect the expression level of cellular reactive oxygen species(ROS).Results On the third day of culture,the proliferation activity of the primary osteoblasts in the control and propofol groups was significantly better than that in the high glucose group(P<0.05).Compared with the control group,the apoptosis rate of primary osteoblasts,Caspase-3 protein,and ROS fluorescence levels in the high glucose group were significantly increased(P<0.05).Compared with the high glucose group,the apoptosis rate of primary osteoblasts,Caspase-3 protein,and ROS fluorescence levels in the propofol group were significantly reduced(P<0.05).Conclusion High glucose can cause oxidative stress injury and increase apoptosis in primary rat osteoblasts.Propofol can decrease oxidative stress in primary rat osteoblasts caused by high glucose,reduce apoptosis and promote cell proliferation,which provides a new avenue for the treatment of skeletal system diseases under hyperglycemia.
作者
冯大鹏
石磊
祝勇刚
吕昌伟
蒋鹏
FENG Da-peng;SHI Lei;ZHU Yong-gang;LYU Chang-wei;JIANG Peng(Department of Critical Care Medicine,Xi'an First Hospital,Xi'an 710002,China;Department of Osteology,.Xijing Hospital,Military Medical University of PLA Air Force,Xi'an 710032,China;Department of Anesthesiology,Xijing Hospital,Military Medical University of PLA Air Force,Xi'an 710032,China;Department of Osteology,Xi'an Third Hospital,Xi'an 710018,China)
出处
《解放军医药杂志》
CAS
2020年第3期15-19,共5页
Medical & Pharmaceutical Journal of Chinese People’s Liberation Army
基金
国家自然科学基金项目(81772328) 陕西省重点研发计划项目(2019SF 194)。
关键词
丙泊酚
高糖
氧化性应激
成骨细胞
细胞凋亡
细胞增殖
活性氧
Propofol
High glucose
Oxidative stress
Osteoblasts
Apoptosis
Cell proliferation
Reactive oxygen species
