摘要
目的探讨长链非编码肺腺癌转移相关转录子1(long non-coding metastasis-associated lung adenocarcinoma transcript 1,MALAT1)在肝癌中的表达情况及其对化疗药物敏感性的影响。方法采用实时荧光定量PCR检测92例肝癌组织及其对应癌旁正常组织中lnc-MALAT1的表达量;分析lnc-MALAT1表达量与肝癌患者临床特征和总生存期的关系。使用HpG2细胞转染MALAT1过表达质粒,转染空载体作为对照组。通过CCK-8实验测定过表达lnc-MALAT1后对细胞增殖的影响;通过化疗药物敏感性实验测定过表达lnc-MALAT1后细胞对化疗药物的敏感性影响。结果lnc-MALAT1在肝癌组织中高表达(P<0.01);其高表达在肝癌患者临床分期(P=0.002)、原发病灶(P=0.027)、淋巴结转移(P=0.026)、远端转移(P=0.004)、化疗(P=0.012)及乙肝表面抗原(hepatitis B surface antigen,HBsAg;P=0.039)方面比较,差异均具有统计学意义。高表达患者总生存时间短于低表达患者[(26.69±16.02)个月vs(37.14±15.77)个月,P=0.027]。转染48 h后,过表达lnc-MALAT1能够促进HpG2细胞增殖(P<0.05)。使用≥0.01 ng/mL顺铂和≥0.1 ng/mL氟尿嘧啶均发现过表达MALAT1能够降低HpG2细胞对以上2种药物的敏感性(均P<0.01)。结论lnc-MALAT1在肝癌中高表达,能够缩短患者生存期,并降低肝癌细胞对化疗药物的敏感性。
Objective To detect the expression pattern of long non-coding metastasis-associated lung adenocarcinoma transcript 1(lnc-MALAT1)in liver carcinoma and its effect on the sensitivity of chemotherapy.Methods Real-time quantitative PCR was used to detect the expression of lnc-MALAT1 in liver cancer tissues and normal tissues adjacent to the cancer.The relationship between the expression of lnc-MALAT1 and the clinical characteristics and overall survival of liver cancer patients were analyzed.HpG2 cells were used to transfect the MALAT1 overexpression plasmid,and the empty vector was used as a control group.The effect of overexpression of lnc-MALAT1 on cell proliferation was measured by CCK-8 experiment,and the sensitivity of chemotherapy after overexpression of lnc-MALAT1 on HpG2 cells was determined by chemotherapeutic drug sensitivity experiment.Results lnc-MALAT1 was highly expressed in liver cancer tissues(P<0.01);liver cancer patients with an overexpression of lnc-MALAT1 had a shorter overall survival[(26.69±16.02)months vs(37.14±15.77)months,P=0.027].The highly expressed lnc-MALAT1 was correlated with primary lesions(P=0.027),lymph node metastasis(P=0.002),distant metastasis(P=0.004),chemotherapy(P=0.012),and hepatitis B surface antigen(HBsAg,P=0.039)of liver cancer patients.48 h after transfection of HpG2 cells,the overexpression of lnc-MALAT1 promoted the proliferation(P<0.05)and inhibited the sensitivity of chemotherapy(≥0.01 ng/mL cisplatin or≥0.1 ng/mL 5-FU,all P<0.01).Conclusions lnc-MALAT1 is highly expressed in liver cancer tissues,which can shorten the overall survival of liver cancer patients,and reduce the sensitivity of liver cancer cells to chemotherapy drugs.
作者
赵一鉴
刘敏
荆冠军
Zhao Yijian;Liu Min;Jing Guanjun(Department of Oncology,Shangluo Central Hospital,Shangluo 726000,China;Department of Oncology,Affiliated Hospital of Yan’an University,Yan'an 716000,China)
出处
《实用肿瘤杂志》
CAS
2020年第1期53-58,共6页
Journal of Practical Oncology
基金
陕西省卫计委科技计划(2016D065)。
