摘要
目的制备超活化富血小板裂解液(super-activated platelet lysate,sPL),研究其对雄激素性脱发(androgenetic alopecia,AGA)小鼠模型的毛囊再生的影响,为sPL治疗AGA的临床研究提供基础。方法使用超滤离心从全血中分离富血小板血浆(platelet-rich plasma,PRP),用冻融方式将血小板裂解制备血小板裂解液(platelet lysate,PL)。用CaCl2结合肝素激活PRP中的血小板,冻融裂解血小板的同时用温度浮动控制方法去除纤维蛋白原,获得sPL。通过ELISA分析PRP、PL及sPL中毛囊再生相关因子含量。用丙酸睾酮注射建立C57bl/6小鼠AGA模型后,用sPL进行治疗,取小鼠背部皮肤,苏木素伊红染色判断sPL对毛囊再生的作用。结果经超滤离心及激活获得的sPL中,数种毛囊再生相关因子较PRP及PL均显著增加。AGA小鼠经sPL治疗后毛发再生明显,同时毛囊密度恢复至对照组水平(t=1.149,P=0.2699)。结论相对于PRP与PL,sPL制备方法可获得更高的生长因子含量,且sPL对AGA小鼠具有显著的毛囊再生作用。
ObjectiveTo prepare super-activated platelet lysate(sPL)and study its effect on hair follicle regeneration in a mouse model of androgenetic alopecia(AGA),providing basis for AGA treatment research.MethodsPlatelet-rich plasma(PRP)was isolated from whole blood by ultrafiltration centrifugation.Concentrated platelets were frozen and thawed to obtain platelet lysate(PL).To prepare sPL,first platelet activation was performed by addition of CaCl2 in combination with heparin,then the sample was completely lysed by freeze-thaw method and fibrinogen was depleted by temperature controlled clotting procedure.Hair follicle regeneration-related growth factors in PRP,PL and sPL were measured by ELISA.C57bl/6 AGA mouse model was established by administration of testosterone propionate.Hair follicle regeneration after treatment of sPL was revealed by hemotoxilin and eosin staining.ResultsThe amount of several hair regeneration-related growth factors in sPL obtained with ultrafiltration centrifugation and platelet activation was significantly higher than that in PRP and PL.AGA mice evidently regrowed hair after sPL treatment,and hair follicle density increased to the level of control mice(t=1.149,P=0.2699).ConclusionsComparing with PRP and PL,sPL preparation can result in higher growth factor concentrations,and sPL has significant hair follicle regeneration effect on AGA mouse model.
作者
刘春香
王博昊
张怡
Liu Chunxiang;Wang Bohao;Zhang Yi(National and Local Joint Stem Cell Research&Engineering Center for Aging Diseases,Heilongjiang Tianqing Stem Cell Co.,Ltd.,Harbin 150028,China)
出处
《国际生物制品学杂志》
CAS
2020年第1期16-19,共4页
International Journal of Biologicals
关键词
秃发
毛囊再生
超活化富血小板裂解液
Alopecia
Hair follicle regeneration
Super-activated platelet lysate
