期刊文献+

SU11274抑制人子宫内膜癌细胞增殖的分子机制 被引量:2

Study on the molecular mechanism of SU11274 inhibiting the proliferation of human endometrial carcinoma cells
下载PDF
导出
摘要 目的:探索HGF/c-met传导通路抑制剂SU11274抑制子宫内膜癌细胞增殖的分子机制。方法:使用不同浓度SU11274(0.5μmol/L、1.0μmol/L、1.5μmol/L)作用ishikawa和HEC-1B两种细胞株1小时后,加入40 ng/ml的HGF,继续培养48小时,然后使用RT-PCR检测c-met、Survivin、XIAP水平以及Western blot检测细胞中Survivin、XIAP蛋白表达水平。结果:ishikawa细胞c-met低表达,而HEC-1B细胞c-met明显高表达,HEC-1B细胞中c-met mRNA表达量为ishikawa中的2.5倍。SU11274可以使HEC-1B、ishikawa细胞的Survivin、XIAP蛋白表达下调,呈现剂量依赖性,并且在HEC-1B细胞中,该下调作用明显强于ishikawa细胞(P<0.05)。结论:SU11274可以通过下调HGF/c-met传导通路中Survivin、XIAP的表达来抑制人子宫内膜癌细胞增殖。 Objective:To explore the molecular mechanism of SU11274,an inhibitor of HGF/c-met pathway,in inhibiting the proliferation of endometrial cancer cells.Methods:Two different cell lines,ishikawa and HEC-1 B,were treated with different concentrations of SU11274(0.5 μmol/L,1.0 μmol/L,1.5 μmol/L) for 1 hour.40 ng/ml of HGF was added,and culture was continued for 48 hours.The levels of c-met,Survivin and XIAP were detected by RT-PCR and the expression of Survivin and XIAP in the cells was detected by Western blot.Results:The expression of c-met was lower in ishikawa cells,while the expression of c-met was significantly higher in HEC-1 B cells.The expression of c-met mRNA in HEC-1 B cells was 2.5-fold higher than that in ishikawa.SU11274 can down-regulate the expression of Survivin and XIAP proteins in HEC-1 B and ishikawa cells in a dose-dependent manner,and the down-regulation of HEC-1 B cells was significantly stronger than that of ishikawa cells(P<0.05).Conclusion:SU11274 can inhibit the proliferation of human endometrial cancer cells by down-regulating the expression of Survivin and XIAP in HGF/c-met pathway.
作者 雷磊 梁静 刘鹏 张竣 周敏 胡晓君 周明 孟龄婷 李青 Lei Lei;Liang Jing;Liu Peng;Zhang Jun;Zhou Min;Hu Xiaojun;Zhou Ming;Meng Lingting;Li Qing(Shaanxi Province Tumor Hospital,Shaanxi Xi'an 710061,China)
机构地区 陕西省肿瘤医院
出处 《现代肿瘤医学》 CAS 2020年第7期1081-1085,共5页 Journal of Modern Oncology
基金 2017年陕西省科技厅社发项目(编号:2017SF-241)。
关键词 SU11274 分子机制 人子宫内膜癌细胞 SU11274 molecular mechanism human endometrial carcinoma cells
  • 相关文献

参考文献2

二级参考文献25

  • 1George F. VANDE WOUDE.HGF/SF-Met signaling in tumor progression[J].Cell Research,2005,15(1):49-51. 被引量:25
  • 2JonathanS,BerekN.妇科学[M].北京:人民卫生出版社,2008.
  • 3Mendes KN, Nicorici D, Cogdell D, et al. Analysis of sig- naling pathways in 90 cancer cell lines by protein lysate array[J]. Journal of Proteome Research,2007,6(7): 2753- 2767.
  • 4Su HY, Lai HC,Lin YW,et al. Epigenetic silencing of SFRP5 is related to malignant phenotype and chemoresis- tance of ovarian cancer through Wnt signaling pathway[J]. International Journal of Cancer, 2010,127 (3) : 555-567.
  • 5Maulik G,Shrikhande A,Kijima T,et al. Role of the hep- atocyte growth factor receptor, e-met,in oncogenesis and potential for therapeutic inhibition[J]. Cytokine & Growth Factor Reviews, 2002,13 ( 1 ) : 41-59.
  • 6Kammula US, Kuntz E J, Francone TD, et al. Molecular co- expression of the c-met oncogene and hepatocyte growth factor in primary colon cancer predicts tumor stage and clinical outcome [J]. Cancer Letters, 2007,248 (2) : 219- 228.
  • 7Hunter AM,LaCasse EC,Korneluk RG. The inhibitors of apoptosis (IAPs) as cancer targets [J]. Apoptosis, 2007,12 (9) : 1543-1568.
  • 8Takeuchi H, Kim J, Fujimoto A, et al. X-linked inhibitor of apoptosis protein expression level in colorectal cancer is regulated by hepatocyte growth Factor/C-met pathway via Akt signaling[J]. Clinical Cancer Research,2005,11 (21) : 7621-7628.
  • 9Guseva NV, Dessus-Babus SC, Whittimore JD, et al. Char- acterization of estrogen-responsive epithelial cell lines and their infectivity by genital Chlamydia traehomatis[J]. Microbes and Infection, 2005,7 (15) : 1469-1481.
  • 10Ma PC, Maulik G, Christensen J, et al. c-met: Structure, functions and potential for therapeutic inhibition[J]. Can- cer and Metastasis Reviews, 2003,22(4) : 309-325.

共引文献3

同被引文献32

引证文献2

二级引证文献7

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部