摘要
采用开顶箱气室(OTCs)研究不同臭氧浓度(80和160 nmol·mol-1)及增温(比对照的大气温度增加2℃)对园林观赏植物醉蝶花(Cleome spinosa)氧化及抗氧化特性的影响。结果表明:(1)经过160 nmol·mol-1高浓度臭氧急性熏蒸7 d后,醉蝶花叶片出现了明显的伤害症状,叶片表面呈现较大面积的漂白褪绿斑块,其他处理下无明显可见伤害症状;(2)与对照相比,高浓度臭氧(160 nmol·mol-1)处理下醉蝶花叶片丙二醛(MDA)含量、相对电导率、过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性均分别显著升高175.7%、259.9%、111.4%、54.3%(P<0.05),而超氧自由基(O2-·)产生速率显著降低67%(P<0.05);臭氧浓度80 nmol·mol-1处理下,MDA含量显著升高65.5%(P<0.05),CAT活性显著下降65.9%(P<0.05),说明醉蝶花在臭氧浓度80 nmol·mol-1左右时具有较高的臭氧耐受性,而臭氧浓度160 nmol·mol-1左右时植物叶片遭受臭氧胁迫,主要通过增强POD和SOD等抗氧化酶活性来抵御氧化伤害;(3)单独增温导致醉蝶花叶片的MDA含量和相对电导率显著升高62.1%和58.1%(P<0.05),但O2-·产生速率和CAT活性显著低于对照(P<0.05),分别降低了28.8%和71.1%,POD和SOD活性与对照相比差别不大,可能是增温使植物自身代谢增强产生这种现象;(4)与单独臭氧处理相比(80 nmol·mol-1),臭氧(80 nmol·mol-1)增温复合处理下植物叶片CAT、POD、SOD均分别显著升高141.7%、72.1%、107.9%(P<0.05);O2-·产生速率显著下降51%(P<0.05),说明臭氧和增温复合处理使醉蝶花遭受的臭氧胁迫加剧。该研究结果可为我国应对高浓度臭氧污染的突发急性事件下的园林及城市绿化植物选择和栽培提供科学依据。
An experiment was conducted to examine the oxidation and anti-oxidizing response in leaves of Cleome spinosa,a garden ornamental plant,exposed to different ozone concentrations(80 and 160 nmol·mol-1)and increasing temperature treatments(2℃higher than control)in open-top chambers(OTCs).The results showed that:(1)A visible leaf injury was observed after seven days with gas fumigation by elevated O3(160 nmol·mol-1),and a large number of bleaching and chlorotic patches appeared on the leaves,whereas no visible injury was observedunder 80 nmol·mol-1.(2)Under high O3 concentration(160 nmol·mol-1),malondialdehyde content(MDA),relative conductivity,peroxidase(POD)activity and superoxide dismutase(SOD)activity of C.spinosa leaves significantly increased by 175.7%,259.9%,111.4%,54.3%,respectively(P<0.05),while superoxide radical(O2-·)production rate was significantly reduced by 67%(P<0.05).Under the treatment of ozone concentration of 80 nmol·mol-1,MDA content increased significantly by 65.5%(P<0.05),catalase(CAT)activity decreased significantly by 65.9%(P<0.05),indicating that C.spinosa had high O3 tolerance ability at the concentration of 80 nmol·mol-1 O3.Under the O3 concentration of 160 nmol·mol-1 O3,plant leaves suffered O3 stress,and they resisted oxidative damage mainly by increasing antioxidant activity(POD and SOD).(3)Under single increasing temperature(IT),MDA content and relative conductivity of C.spinosa leaves increased significantly by 62.1%and 58.1%(P<0.05)respectively,but the O2-·production rate and CAT activity were 28.8%and 71.1%lower than the control respectively(both P<0.05),POD and SOD activities were not significantly different from the control,which may be attributed to enhanced plant metabolism due to higher temperature.(4)Compared with 80 nmol·mol-1 O3 alone,CAT,POD and SOD activities under the combination of increasing temperature and elevated O3(80 nmol·mol-1)significantly increased by141.7%,72.1%and 107.9%,respectively(P<0.05);O2-·production rate significantly decreased by 51.0%(P<0.05),indicating that the combined treatment of O3 and increasing temperature exacerbated the O3 stress suffered by C.spinosa.These findings will provide a scientific basis for the selection and cultivation of plant species in gardens and urban greening in China under the sudden acute events of high-concentration ozone pollution.
作者
秦子晴
徐胜
齐淑艳
陈玮
何兴元
王义婧
QIN Zi-qing;XU Sheng;QI Shu-yan;CHEN Wei;HE Xing-yuan;WANG Yi-jing(Shenyang University,Shenyang 110044,China;Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;Key Laboratory of Forest Ecology and Management,Chinese Academy of Sciences,Shenyang 110016,China;University of Chinese Academy of Sciences,Beijing 100049,China;Shengyang Arboretum,Chinese Academy of Sciences,Shenyang 110016,China)
出处
《生态学杂志》
CAS
CSCD
北大核心
2020年第3期830-837,共8页
Chinese Journal of Ecology
基金
国家自然科学基金项目(41675153,31870458,31270518,31170573)
国家重点研发项目(2016YFC0500306)资助。
关键词
OTC模拟
高浓度臭氧
增温
醉蝶花
抗氧化酶活性
OTC simulation
elevated O3concentration
increasing temperature
Cleome spinosa
antioxidant enzyme activity
