摘要
含氨基酸水溶肥是一种新型绿色肥料,具有肥效快、无残留、改善作物品质和提高作物抗逆性等优点。将其作为有机载体制备菌肥,对芽孢杆菌生长和繁殖是否具有影响,尚未见报道。本研究分析不同浓度的"绿农林"含氨基酸水溶肥溶液对芽孢杆菌菌株X05和QB61生长繁殖、拮抗代谢物产生以及对番茄、香蕉生长和香蕉枯萎病防效的影响。结果表明:在1~50 g/L供试含氨基酸水溶肥溶液中培养24 h,芽孢杆菌X05和QB61细胞数量增长为初始菌量的86~2900倍,而在清水中培养24 h,细胞数量未发生明显变化;X05和QB61在50 g/L的含氨基酸水溶肥溶液中培养48 h,上清液对香蕉枯萎病菌的生长具有显著的抑制作用,而在其他浓度的含氨基酸水溶肥溶液和清水中培养48 h,上清液均无抑制作用;与单独施用芽孢杆菌X05或QB61处理相比,施用添加芽孢杆菌X05或QB61的含氨基酸水溶肥处理的番茄和香蕉幼苗生长显著增强,同时香蕉枯萎病发病指数分别降低33.4%和32.2%。以上结果表明,供试氨基酸水溶肥可促进芽孢杆菌X05和QB61的生长和繁殖,并使其产生拮抗代谢物,两者混合施用对番茄和香蕉具有更好的促生效果,并可提高芽孢杆菌对香蕉枯萎病的防效。因此,供试的含氨基酸水溶肥与上述的芽孢杆菌组合具有良好的应用前景。
A water-soluble fertilizer containing amino acids is a new type of green fertilizer, which has many advantages including high efficacy, no residue, potential to improve crop quality and resistance to stress. It can be used as a carrier to make microbial fertilizers. However, whether it influences the growth and reproduction of Bacillus strains has not been reported. We analyzed the impact of the solution of the water-soluble fertilizer containing amino acids(WFA) ‘Lvnonglin’ on the growth and reproduction of the Bacillus strains X05 and QB61, production of antagonistic metabolites, and plant growth promotion of tomato and banana, control efficiency of wilt disease of banana. The cells of Bacillus strains X05 and QB61 increased to 86-2900 fold of the initial inoculation number after 24 h cultivation in 1-50 g/L of the WFA solution, while that had no change in other concentration of the WFA solution and water(control). The supernatant that obtained from the culture of the Bacillus strains X05 and QB61 in 50 g/L of the WFA solution for 48 h showed significant inhibition on the growth of mycelial growth of the fungal pathogen Fusarium oxysporum f. sp. cubense, while the supernatant from the cultures of X05 and QB61 in other concentrations of the WFA solution and water(control) had no inhibition effect. Additionally, in contrast to th control plants(applied with the solution of the Bacillus strain X05 or QB61), the plant growth promotion of banana and tomato seedlings applied with the solution of WFA inoculated with X05 or QB61 showed to be significantly enhanced, and banana plantlets applied with WFA inoculated with QB61 and X05 showed 33.4% and 32.2% reduction in disease index of wilt disease of banana compared to those applied with the Bacillus strains QB61 and X05, respectively. These imply that WFA ‘Lvnonglin’ can promote the growth and reproduction of Bacillus strains X05 and QB61, and enable them to produce antagonistic metabolites. Meanwhile, the application with the combination of WFA and Bacillus strains can promote the plant growth of banana and tomato, and suppress the wilt disease of banana more efficiently. Thus, the combination of WFA and Bacillus strains X05 and QB61 has a good application prospect.
作者
郭立佳
汪军
杨腊英
梁昌聪
周游
刘磊
黄俊生
GUO Lijia;WANG Jun;YANG Laying;LIANG Changcong;ZHOU You;LIU Lei;HUANG Junsheng(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Pests Comprehensive Governance for Tropical Crops,Ministry of Agriculture and Rural Affairs,Haikou,Hainan 571101,China)
出处
《热带作物学报》
CSCD
北大核心
2020年第3期549-555,共7页
Chinese Journal of Tropical Crops
基金
国家重点研发计划项目(No.2017YFD0201106)
海南自然科学基金创新研究团队项目(No.2017CXTD016)
广西创新驱动发展专项资金项目(桂科AA18118011-2)。
关键词
香蕉
番茄
尖孢镰刀菌古巴专化型
代谢物
促生长
banana
tomato
Fusarium oxysporum f.sp.cubense
metabolite
growth promotion
