摘要
目的快速建立免疫力低下的KM小鼠动物模型,用简单快速的方法鉴定动物模型成立。方法环磷酰胺经口灌胃,剂量为100 mg/kg;氢化可的松经口灌胃,剂量为80 mg/kg;60%乙醇溶液经口灌胃,剂量为20m L/Kg;UVA紫外线皮肤照射(长波),剂量为10 J/cm^2,各组连续3 d给药处理。分别测定小鼠试验前后体重,测定小鼠血清中的IgG、IgA、IgM的含量,用流式细胞仪测定小鼠外周血B淋巴细胞CD19、T淋巴细胞CD3的含量,用全自动血液分析仪分析小鼠外周血血细胞情况。结果环磷酰胺组和氢化可的松组的KM小鼠免疫力低下模型成立;60%乙醇组和紫外线照射组免疫力低下模型不成立,需进一步试验探索。测定小鼠体重,检测小鼠血清中的IgG、IgA、IgM的含量和小鼠外周血B、T淋巴细胞以及小鼠血常规分析,可以做为KM小鼠免疫力低下的模型成立的参考检测指标。结论连续经口灌胃3 d 100 mg/Kg的环磷酰胺建立的KM小鼠免疫力低下的动物模型是成立的。从各种检测指标简易程度上来看,体重、血清免疫球蛋白含量、血常规这三个指标简单易得,检测成本低廉且时间周期短,检出有效率高,可作为日常检测工作的动物模型建立确认的指标。
Objective Animal models of low immunity were established in mice using a simple and rapid method.Methods Cyclophosphamide was administered orally at a dose of 100 mg/Kg,hydrocortisone was administered orally at a dose of 80 mg/Kg,a 60% ethanol solution was administered orally at a dose of 20 ml/kg,and skin was irradiated by UVA( long wave) at a dose of 10 J/cm^2. Each group was treated with medicine for 3 consecutive days. The body weight of mice was measured before and after the test,and the levels of IgG,IgA,and IgM in the serum of mice was determined. In mice,the proportions of CD19+ B cells in peripheral blood lymphocytes and CD3+ T cells in peripheral blood lymphocytes were measured by flow cytometry. The peripheral blood cells of mice were analyzed by an automatic hematology analyzer.Results Immunosuppressive mouse models using cyclophosphamide or hydrocortisone were established.Immunosuppressive models using 60% ethanol or ultraviolet irradiation were not established,and require further study. The body weight of mice,the levels of IgG,IgA,and IgM in the serum,B and T lymphocytes in peripheral blood,and the routine analysis of blood in mice can be used as reference indexes for the establishment of models of low immunity in mice.Conclusions A model of immunosuppression established in mice by the oral administration of cyclophosphamide at 100 mg/kg for 3 days was established. Regarding the various detection indexes,body weight,serum immunoglobulin content,and routine blood analysis are simple to perform,rapid,and of low cost with a high detection rate. These can be used as indexes for the routine detection of established animal models.
作者
田嘉军
黄聘和
岳茜岚
何其励
TIAN Jiajun;HUANG Pinghe;YUE Qianlan;HE Qili(Sichuan provincial disease prevention and control center,Chengdu 610041,China)
出处
《中国比较医学杂志》
CAS
北大核心
2020年第3期77-82,共6页
Chinese Journal of Comparative Medicine
基金
四川省疾病预防控制中心自立科研课题(ZX201616)。
关键词
免疫力低下
动物模型
快速检测
immunosuppressive
animal model
rapid detection
