摘要
目的研究单核细胞增生李斯特菌(Lm)感染致Bewo细胞炎症因子表达及迁移能力的变化,并探讨其可能的机制。方法Lm以MOI=10感染Bewo细胞,实时荧光定量PCR(qRT-PCR)检测Bewo细胞炎症因子IL-1β、IL-6及TNF-α的mRNA水平;划痕试验及Transwell试验检测Bewo细胞的迁移能力;Western Blot检测Bewo细胞迁移相关蛋白(MMP2,MMP9,TIMP-1)以及MAPK家族蛋白(p-p38MAPK,p38MAPK,p-ERK1/2,ERK1/2)的表达水平。结果Lm感染Bewo细胞后炎症因子IL-1β、IL-6及TNF-α的mRNA水平与感染1 h相比均升高。Western Blot结果表明,随着感染时间的延长,迁移相关蛋白MMP2逐渐升高;MMP9和TIMP-1变化趋势一致,先上升后下降;Lm感染致MAPK家族p38MAPK及ERK1/2蛋白磷酸化水平升高。结论Lm感染Bewo细胞后导致细胞迁移能力增强,MMP2在调控细胞迁移能力的变化中起主要作用,Lm感染可以激活Bewo细胞MAPK家族p38MAPK及ERK1/2信号通路。
The aim of this study was to investigate the changes of the inflammation and migration ability in Bewo cells induced by Listeria monocytogenes(Lm)infection and to explore its molecular mechanism.Bewo cells were infected by Lm at MOI=10,and the mRNA levels of inflammatory factors of IL-1β,IL-6 and TNF-αin Bewo cells were detected by quantitative real-time PCR(qRT-PCR),wounding healing and transwell chamber were used to detect the migration ability of Bewo cells,Western Blot was used to detect Bewo cell migration-associated proteins(MMP2,MMP9,TIMP-1)and MAPK proteins(p-p38MAPK,p38MAPK,p-ERK1/2,ERK1/2).The results showed that the mRNA levels of inflammatory cytokines IL-1β,IL-6,TNF-αwere increased after Lm infection,also the expressions of MMP2 and p-p38MAPK protein were increased respectively,however,the expressions of MMP9,TIMP-1 and p-ERK1/2 were rose first and then decreased.Among these results,we draw a conclusion that enhanced migration ability may be regulated by MMP2.P38MAPK and ERK1/2 signaling pathway play a major role in Lm-infected Bewo cells.
作者
朱昱蓉
史倩
卢叶
凌薇
袁琳
杨诗娴
王蓓蕾
姜旭淦
陈盛霞
ZHU Yu-rong;SHI Qian;LU Ye;LING Wei;YUAN Lin;YANG Shi-xian;WANG Bei-lei;JIANG Xu-gan;CHEN Sheng-xia(School of Medicine,Jiangsu University,Zhenjiang 212013,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2020年第2期94-99,共6页
Chinese Journal of Zoonoses
基金
中国科技部基础平台项目资助(No.平台-TDRC-22)。
