摘要
目的探讨营养包对早期婴幼儿肠道菌群的影响。方法选择"2018年早期儿童营养包干预远期效果项目"中覆盖县河南嵩县和未覆盖县对照县汝阳县,采用按规模大小成比例的概率抽样法抽中3~5个样本村,对抽中样本村中所有6~24月龄婴幼儿采用随机等距抽样方法,最终抽取118例婴幼儿为调查对象,其中嵩县60例,汝阳县58例。于2018年9月体检时收集调查对象粪便,采用高通量测序技术对肠道菌群构成与丰度进行分析比较。结果12~24月龄婴幼儿营养包干预组的肠道菌群Alpha多样性显著高于对照组;6~24月龄婴幼儿营养包干预组厚壁菌门呈上升趋势;6~11月龄婴幼儿营养包干预组普雷沃氏菌(Prevotella)丰度显著低于对照组;12~17月龄婴幼儿营养包干预组粪杆菌属(Faecalibacterium)和拟杆菌属(Bacteroides)丰度显著高于对照组,双歧杆菌属(Bifidobacterium)和普雷沃氏菌丰度显著低于对照组;18~24月龄婴幼儿营养包干预组乳杆菌属(Lactobacillus)丰度显著低于对照组。结论营养包可改善肠道菌群多样性,对促进婴幼儿肠道菌群平衡发挥重要作用。
OBJECTIVE To investigate the effect of nutrition package on intestinal flora of infants aged 6-24 months.METHODS A total of 118 infants in Songxian County and Ruyang County of Henan Province,60 infants in Songxian County with nutrition package coverage,and 58 infants in Ruyang County of the control county were selected for the long-term effects of early childhood nutrition package intervention project in 2018.All infant faeces were collected,and the composition and abundance of intestinal flora were analyzed and compared by high-throughput sequencing technology.RESULTS The Alpha diversity of intestinal flora in the nutrition package intervention group was significantly higher than that in the control group.Firmicutes were on the rise in the nutrition package intervention group of infants aged 6-24 months.The abundance of Prevotella in the intervention group was significantly lower than that in the control group.The abundance of Faecalibacterium and Bacteroides in the nutrition package treatment group for infants aged 12 to 17 months was significantly higher than that of the control group,while the abundance of Bifidobacterium and Prevotella was significantly lower than that of the control group.Lactobacillus was significantly less abundant in the 18-24 month old infant nutrition package intervention group than in the control group.CONCLUSION Nutrition package can improve the diversity of intestinal flora,also play an important role in promoting the balance of intestinal flora of infants.
作者
王丽媛
宫照龙
霍军生
卓勤
秦文
杨倬
王晶波
沈葹
Wang Liyuan;Gong Zhaolong;Huo Junsheng;Zhuo Qin;Qin Wen;Yang Zhuo;Wang Jingbo;Shen Shi(National Institute for Nutrition and Health,China CDC,Key Laboratory of Trace Element Nutrition,National Health Commission of the People’s Republic of China,Beijing 100050,China)
出处
《卫生研究》
CAS
CSCD
北大核心
2020年第2期233-237,共5页
Journal of Hygiene Research
基金
早期儿童营养包干预长期营养健康作用评估项目(2018-2020)。
关键词
高通量测序
营养包
婴幼儿
肠道菌群
high throughput sequencing
nutrition package
infants
intestinal flora
