摘要
背景:力竭性运动引起大鼠大脑组织发生炎症反应所涉及的生理病理机制仍是十分复杂。研究表明,葛根总黄酮具有抗氧化、改善脑外伤神经和保护心脑血管等作用。目的:探讨葛根总黄酮对力竭性运动大鼠脑组织炎症细胞因子及信号传导和转录活化因子3表达的影响。方法:将50只SD雄性大鼠随机分为安静对照组、运动对照组及葛根总黄酮低、中、高剂量组。除安静对照组外,其余各组进行为期6周的运动训练,于6周末即最后一次训练达到力竭。葛根总黄酮低、中和高剂量组大鼠均在运动前半小时灌胃50,100,200 mg/kg的葛根总黄酮,1次/d,直到实验结束。通过ELISA法测定大鼠血清及脑组织肿瘤坏死因子α、白细胞介素8、白细胞介素1β和白细胞介素10的水平,并采用RT-PCR和Western Blot法检测大鼠脑组织信号传导和转录活化因子3的表达。实验方案经广西师范大学动物实验伦理委员会批准(批准号为GXMU201703049)结果与结论:①运动对照组大鼠血清及脑组织肿瘤坏死因子α、白细胞介素8、白细胞介素10和白细胞介素1β水平均高于安静对照组(P<0.01);其中葛根总黄酮中、高剂量组大鼠血清与脑组织肿瘤坏死因子α和白细胞介素10水平均低于运动对照组(P<0.01);葛根总黄酮低、中和高剂量组大鼠血清与脑组织白细胞介素8和白细胞介素1β水平均低于运动对照组(P<0.01或P<0.05);②运动对照组大鼠脑组织信号传导和转录活化因子3 mRNA及蛋白表达均高于安静对照组(P<0.01);葛根总黄酮低、中和高剂量组大鼠脑组织信号传导和转录活化因子3 mRNA及蛋白表达均低于运动对照组(P<0.01);③结果说明,力竭性运动引起大鼠脑组织发生炎症反应和上调了信号传导和转录活化因子3表达水平,葛根总黄酮具有调节机体内脑组织细胞因子信号传导和转录活化因子3表达以及抑制脑组织的炎症反应,从而达到保护脑组织损伤的作用。
BACKGROUND:The physiological and pathological mechanisms underlying the inflammatory response of rat brain tissue caused by exhaustive exercise are very complicated.Studies have shown that Pueraria total flavonoids have anti-oxidative,neuroprotective,and cardio-cerebrovascular protective effects against brain injury.OBJECTIVE:To investigate the effect of Pueraria total flavone on inflammatory cytokines and signal transducer and activator of transcription-3(STAT3)expression in rat brain tissue after exhaustive exercise.METHODS:Fifty healthy male Sprague-Dawley rats were randomly divided into a quiet control group,an exercise control group,low,middle,high dose Pueraria total flavone groups.Each training group conducted a 6-week sports training.At the end of 6-week exercise,the rats were confirmed to be exhausted.The rats were intragastrically administered 50,100 and 200 mg/kg Pueraria total flavone in low,middle and high dose groups,respectively.Administration in each Pueraria total flavone group began at 30 minutes before exercise,once a day,and ended until the completion of the experiment.The activities of tumor necrosis factor-α,interleukin-8,interleukin-1βand interleukin-10 in serum and brain tissue of rats were determined by ELISA.The expression of STAT3 in rat brain tissue was detected by RT-PCR and western blot.The study protocol was approved by the Animal Ethics Committee of Guangxi Normal University(approval No.GXMU201703049).RESULTS AND CONCLUSION:The levels of tumor necrosis factor-α,interleukin-8,interleukin-1βand interleukin-10 in serum and brain tissue of exercise control rats were higher than those in the quiet control group(P<0.01).The levels of tumor necrosis factor-αand interleukin-10 in serum and brain tissue of rats with middle and high dose of Pueraria total flavone were significantly lower than those in the exercise control group(P<0.01).The levels of interleukin-8 and interleukin-1βin serum and brain tissue of rats with low,middle and high dose of Pueraria total flavone were significantly lower than those in the exercise control group(P<0.01 or P<0.05).The expression levels of STAT3 mRNA and protein in brain tissue of exercise control rats were significantly higher than those in the quiet control group(P<0.01).The expression levels of STAT3 mRNA and protein in the brain tissue of rats with low,middle and high doses of Pueraria total flavone were significantly higher than those in the quiet control group(P<0.01).To conclude,exhaustive exercise can cause inflammatory reaction and up-regulate STAT3 expression in rat brain tissue.The total flavonoids of Puerariae can regulate the expression of STAT3 in brain tissues and inhibit the inflammatory response of brain tissue,thus protecting damaged brain tissue.
作者
蒋宇凌
莫伟彬
唐健
李敏华
Jiang Yuling;Mo Weibin;Tang Jian;Li Minhua(Department of Physical Education,Guangxi University of Finance and Economics,Nanning 530003,Guangxi Zhuang Autonomous Region,China;School of Physical Education,Guangxi Normal University,Guilin 541004,Guangxi Zhuang Autonomous Region,China;State Key Laboratory of Medicinal Resources Chemistry and Molecular Engineering,Guilin 541004,Guangxi Zhuang Autonomous Region,China)
出处
《中国组织工程研究》
CAS
北大核心
2020年第23期3678-3684,共7页
Chinese Journal of Tissue Engineering Research
基金
国家自然科学基金资助项目(31460232)
广西教育厅科研项目(2018KY0090),项目负责人:莫伟彬
教育发展基金会“教师成长基金”项目(EDF2016005),项目负责人:莫伟彬。
