摘要
新型冠状病毒(SARS-CoV-2)正在世界范围内暴发流行,导致人类严重呼吸道疾病且致死率高,引起全球的高度关注。该病毒造成的新型冠状病毒肺炎(COVID-19)尚无特效治疗药物,早期诊断是当前主要的防控措施。依据《新型冠状病毒肺炎诊疗方案(试行第七版)》,病毒核酸检测阳性、基因测序高度同源或血清学抗体检测阳性均可确诊SARS-CoV-2感染,然而各种检测方法均存在一定的假阴性率和潜在的假阳性结果。本文对目前正在应用和报道的SARS-CoV-2实验室检测方法,如病毒分离、二代测序技术、反转录-聚合酶链式反应、恒温核酸扩增技术、基因芯片和抗体检测等进行综述,并探讨各种方法的优缺点,为优化SARS-CoV-2感染诊断提供参考。
The outbreak of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)all over the world leads to serious respiratory diseases and high mortality,which arouses wide concern.No specific treatment for coronavirus disease 2019(COVID-19)caused by the virus has been found,so early diagnosis is the main prevention and control measure at present.According to the COVID-19 Diagnosis and Treatment Plan(Seventh Trial),Being positive in nucleic acid testing or in antibody testing,or being highly homologous in gene sequencing can confirm SARS-CoV-2 infection.However,all these detection methods have potential false negative and false positive results.This article summarizes the SARS-CoV-2 laboratory testing methods that are being applied and studied at present,such as virus isolation,second generation sequencing,reverse transcription-polymerase chain reaction,nucleic acid isothermal amplification,gene chip and antibody detection,and discusses their advantages and disadvantages to optimize the diagnosis of SARS-CoV-2 infection.
作者
张友春
汪睿(综述)
朱永良(审校)
ZHANG Youchun;WANG Rui;ZHU Yongliang(Maternal and Child Health Hospital of Chun'an,Chun'an,Zhejiang 311700,China;不详)
出处
《预防医学》
2020年第4期361-365,共5页
CHINA PREVENTIVE MEDICINE JOURNAL
关键词
新型冠状病毒
病毒分离
核酸检测
抗体检测
severe acute respiratory syndrome coronavirus 2
virus isolation
nucleic acid detection
serological detection
