摘要
目的:观察艾灸"足三里"穴对疲劳大鼠外周和中枢白细胞介素(IL)-6、IL-10及IL-10/IL-6的影响,探讨艾灸缓解疲劳的调节机制。方法:SD大鼠随机分为正常组、模型组、艾灸组,每组7只。以21 d负重力竭游泳法制备疲劳大鼠模型。艾灸组大鼠造模第1天用小艾炷灸双侧"足三里",隔日治疗1次,每次每穴艾灸3壮,治疗11次。观测大鼠一般状态和体质量,用酶联免疫吸附法检测血清、腓肠肌与额叶皮层的IL-6、IL-10水平,并计算IL-10/IL-6比值。结果:造模第7、14、21天,模型组体质量较正常组显著降低(P<0.05)。造模第14、21天,艾灸组体质量较模型组显著增加(P<0.05)。与正常组比较,模型组血清、腓肠肌及额叶皮层IL-6水平明显升高(P<0.05,P<0.01)、IL-10水平明显降低(P<0.05,P<0.01);模型组血清、额叶皮层IL-10/IL-6明显降低(P<0.01)。与模型组比较,艾灸组腓肠肌和额叶皮层IL-6明显降低(P<0.05);艾灸组血清、额叶皮层IL-10明显升高(P<0.05);艾灸组血清、额叶皮层IL-10/IL-6明显升高(P<0.01)。结论:艾灸可明显缓解运动性疲劳,其作用可能是通过降低外周和中枢促炎因子IL-6、提高抗炎因子IL-10和IL-10/IL-6比值,减轻外周和中枢炎性反应,改善促炎因子及抗炎因子分泌失衡而实现的。
Objective To observe the effect of moxibustion at"Zusanli"(ST36)on peripheral and central IL-6,IL-10 and IL-10/IL-6 changes in fatigue rats,so as to explore its mechanisms underlying relief of fatigue.Methods Twenty-one male SD rats were randomly divided into normal control,model and moxibustion groups with 7 rats in each group.The fatigue model was induced by exhausted weight-loaded swimming,once daily for 21 days.Moxibustion was applied to bilateral ST36 once every other day,for successive 11 times.The overall state and the body weight were observed.The levels of IL-6 and IL-10 in the serum,gastrocnemius and frontal cortex tissues were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with the normal group,the body weight was significantly reduced on the 7th,14th,and 21st day in the model group(P<0.05),and considerably increased on the 14th and 21st day in the moxibustion group relevant to the model group(P<0.05).After modeling,the levels of IL-6 in the serum,gastrocnemius and frontal cortex were significantly increased(P<0.05,P<0.01),while those of IL-10 was obviously decreased(P<0.05,P<0.01)in the model group relevant to the normal group,and ratio of IL-10/IL-6 was obviously decreased in the serum and frontal cortex of the model group relevant to the normal group(P<0.01).After the moxibustion intervention,the levels of IL-10 in the serum and frontal cortex were significantly increased(P<0.05),and ratio of IL-10/IL-6 in the serum and frontal cortex was significantly increased(P<0.01),while the IL-6 contents in the gastrocnemius and frontal cortex were notably decreased in the moxibustion group relevant to the model group(P<0.05).Conclusion Moxibustion can inhibit weight-loaded swimming induced decrease of body weight in fatigue rats,which may be associated with its effects in balancing the levels of peripheral and central pro-inflammatory cytokine IL-6 and anti-inflammatory cytokine IL-10.
作者
赵雅
李天歌
蒲荣
水玲
卢峻
陈英松
ZHAO Ya;LI Tian-ge;PU Rong;SHUI Ling;LU Jun;CHEN Ying-song(School of Acupuncture-moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China;Eighth Medical Center,General Hospital of Chinese People’s Liberation Army,Beijing 100091;Mongolian Medical College,Inner Mongolia Medical University,Huhhot 010110)
出处
《针刺研究》
CAS
CSCD
北大核心
2020年第3期215-219,共5页
Acupuncture Research
基金
国家自然科学基金项目(No.81560802)。
