摘要
目的分离和纯化两色金鸡菊中的黄诺玛苷单体,并研究其抗血小板聚集的作用。方法先用55%乙醇回流提取两色金鸡菊干燥花序得到两色金鸡菊醇提物(ethanol extract,ETE),再用乙酸乙酯萃取得到的水层部位为乙酸乙酯萃取物(acetic ether extracted residuum,AR),AR在经大孔树脂富集,并收集75%乙醇洗脱部位得到醇提纯化物(ethanol extract purification,ETEP),ETEP经聚酰胺柱层析分离得到一个黄酮类组分黄诺玛苷单体;静脉采集健康受试者的血液,随机分为空白对照组(0.9%氯化钠注射液)、ETE组(大900μg·mL^-1、中300μg·mL^-1、小100μg·mL^-1剂量组)、黄诺玛苷组(大90μg·mL^-1、中30μg·mL^-1、小10μg·mL^-1剂量组)、阿司匹林(Acetylsalicylic acidsigma,ASA)组(500μg·mL^-1),建立二磷酸腺苷(adenosine diphosphate,ADP)、凝血酶诱导的血小板聚集模型。依据Born比浊法,通过血小板聚集的抑制率来观察黄诺玛苷的抗血小板聚集。结果从两色金鸡菊花序中成功的分离得到纯度达到92%以上的黄诺玛苷的单体化合物;抗血小板聚集实验通过和空白对照组相比对ADP诱导的血小板聚集有抑制作用的是醇提物的中剂量组、大剂量组和黄诺玛苷大剂量组,差异具有统计学意义(P<0.01);对凝血酶诱导的血小板聚集有抑制作用的是醇提物的中剂量组和黄诺玛苷中剂量组,差异具有统计学意义(P<0.05);在大剂量醇提物组和大剂量的黄诺玛苷组中,凝血酶诱导的血小板聚集受到抑制,差异具有统计学意义(P<0.01)。结论从两色金鸡菊中分离得到的黄诺玛苷单体是抗血小板聚集的主要活性物质之一。
Objective To isolate and purify flavanomarein from Coreopsis tinctoria,and to study its anti-platelet aggregation effect.Methods The dry inflorescence of Coreopsis tinctoria was extracted by refluxing using 55%ethanol to obtain ethanol extract(ETE),and then the aqueous layer from ETE was extracted by ethyl acetate to obtain ethyl acetate extract(AR)after gathering by macroporous resin.Furthermore,Flavanomarein of flavonoids was isolated from ETEP by polyamide chromatography column.The blood sample of healthy subjects was collected via vein and randomly divided into blank control group(saline),ETE group(high dose of 900μg·mL^-1,medium dose of 300μg·mL^-1,low dose of 100μg·mL^-1),flavanomarein group(high dose of 90μg·mL^-1,medium dose of 30μg·mL^-1,low dose of 10μg·mL^-1),aspirin(acetylsalicylic acid,ASA)group(500μg·mL^-1).A platelet aggregation model induced by adenosine diphosphate(ADP)and thrombin was established.Born turbidimetric method was used to determine the platelet aggregation inhibition rate by observing its anti-platelet aggregation effect.Results Flavanomarein with purity of more than 92%was successfully isolated from the dry inflorescence of Coreopsis tinctoria.Compared with the blank control group,the medium and high dose groups of ETE and the high dose group of flavanomarein could inhibit ADP-induced platelet aggregation(P<0.01).Compared with the blank control group,the medium dose group of ETE and the medium dose group of flavanomarein could inhibit thrombin-induced platelet aggregation,and the differences were statistically significant(P<0.01).The high dose of AR group and the high dose of flavanomarein group could inhibit thrombin-induced platelets aggregation,and the difference was statistically significant(P<0.01).Conclusion Flavanomarein in the Coreopsis tinctoria was one of the main active substances for anti-platelet aggregation.
作者
张永威
王敬伟
李新霞
李琳琳
邓凯
骆新
王丽凤
毛新民
ZHANG Yongwei;WANG Jingwei;LI Xinxia;LI Linlin;DENG Kai;LUO Xin;WANG Lifeng;MAO Xinmin(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China;College of Basic Medical,Xinjiang Medical University,Urumqi 830011,China;College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830011, China)
出处
《医药导报》
CAS
北大核心
2020年第4期427-431,共5页
Herald of Medicine
基金
国家自然科学基金资助项目(U1303233)。
关键词
两色金鸡菊
黄诺玛苷
聚酰胺柱层析
抗血小板聚集实验
Coreopsis tinctoria
Flavanomarein
Polyamide column chromatography
Antiplatelet aggregation experiment
