摘要
目的:检测溶酶体相关4次跨膜蛋白B(lysosome-associated protein transmembrane-4 beta,LAPTM4B)-35蛋白在正常涎腺及涎腺腺样囊性癌中的表达水平,探讨其与肿瘤患者临床病理特征的相关性。方法:收集95例涎腺腺样囊性癌和20例正常涎腺组织,以及相关临床病理资料,应用免疫组织化学法检测LAPTM4B-35在正常涎腺组织、涎腺腺样囊性癌的表达水平,卡方检验或Fisher精确检验及多因素Logistic回归分析探讨LAPTM4B-35表达水平与涎腺腺样囊性癌临床病理特征的关系。结果:LAPTM4B-35蛋白在正常涎腺组织不同细胞中呈差异性表达:在腺泡细胞中表达为阴性,在导管和闰管细胞中表达很弱,在纹状管细胞中表达中等。在涎腺腺样囊性癌组织中,48.00%的高级别涎腺腺样囊性癌LAPTM4B-35表达增高,显著高于低级别涎腺腺样囊性癌。此外,LAPTM4B-35高表达与临床分期晚亦呈显著相关。结论:LAPTM4B-35蛋白在正常涎腺组织细胞中呈差异性表达,与涎腺腺样囊性癌的组织学分级和临床分期显著相关。
Objective:This study aims to investigate the expression of LAPTM4 B-35 protein in normal salivary gland and salivary adenoid cystic carcinoma,and to explore the correlation between LAPTM4 B-35 protein expression and clinicopathological characteristics of tumor patients.Methods:The clinical and pathological data of 95 cases of adenoid cystic carcinoma and 20 cases of normal salivary gland were collected.By immunohistochemistry analysis,LAPTM4 B-35 expression was evaluated in salivary adenoid cystic carcinoma tissues and normal salivary tissues.The correlation of LAPTM4 B-35 expression with clinic-pathological parameters was assessed using Chi-square or Fisher’s exact test and Logistic regression analysis.Results:The expression level of LAPTM4 B-35 was variant among different cell types of normal salivary glands.It was expressed fairly low in serous and mucous acini,weak in intercalated duct and excretory duct cells,moderate in secretory/striated ducts.In 48.00%of high grade salivary adenoid cystic carcinoma tested,LAPTM4 B-35 was high expression,which was significantly higher than that in low grade salivary adenoid cystic carcinoma.The expression rate of LAPTM4 B-35 were significantly associated with histological grades and clinical stage.Conclusion:LAPTM4 B-35 protein was differentially expressed in normal salivary gland cells,and expression of LAPTM4 B-35 protein was significantly related to the histological grade and clinical stage of adenoid cystic carcinoma.
作者
范建林
杨建新
乔卫平
刘蔚
FAN Jianlin;YANG Jianxin;QIAO Weiping;LIU Wei(Department of Stomatology,the Second Affiliated Hospital of Soochow University,Suzhou 215004;Department of Stomatology,Suzhou Wuzhong People’s Hospital,Suzhou 215128;Department of Pathology,the First Affiliated Hospital of Soochow University,Suzhou 215006,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2020年第3期390-395,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
苏州市科技发展计划(SYSD2015087)。
