摘要
目的:观察灵芝三萜类化合物(GLT)对砷中毒大鼠脑组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的影响。方法:60只大鼠随机均分为正常对照组(NS 10 m L/kg)、模型组(亚砷酸钠10 mg/kg)和GLT低、中、高剂量组(GLT 0.25、0.5、1.0 g/kg),连续砷染毒90 d后建立砷中毒大鼠模型;观察GLT对砷中毒大鼠脑组织中T-AOC、SOD活性及MDA含量的变化。结果:与正常对照组比较,模型组T-AOC和SOD活力明显降低,MDA含量明显升高(P<0.05);给予GLT干预后,与模型组比较,GLT各剂量组T-AOC、SOD活性明显上升(P<0.05),GLT中、高剂量组MDA含量明显降低(P<0.05)。结论:GLT能够改善砷中毒大鼠的氧化应激损伤,其作用机制可能与升高脑组织中T-AOC、SOD活性及降低MDA含量有关。
Objective:To evaluate anti-oxidative effects of Ganoderma lucidum triterpenes(GLT)on arsenism-induced injury in the brain of rats by detecting the total antioxidant capacity(T-AOC)and superoxide dismutase(SOD)activity and malonaldehyde(MDA)content.Methods:60 rats were randomly divided into 5 groups:the control group(NS 10 mL·kg-1),the model group(sodium arsenite 10 mg·kg-1),GLT low,middle and high dose groups(GLT 0.25,0.5,1.0 g·kg-1).90 days after exposure to arsenic,the rat model of arsenic poisoning was established,and the indexes were detected by kit instructions to observe the changes of T-AOC,SOD activity and MDA content in the brain tissues of arsenic poisoning rats induced by GLT.Results:Compared with the control group,the activities of T-AOC and SOD in the model group decreased significantly,while the content of MDA increased significantly.After the intervention of GLT,compared with the model group,the activities of T-AOC and SOD increased significantly,while the content of MDA decreased significantly.Conclusion:GLT can improve the oxidative stress injury in rats with arsenism,and its mechanism may be related to the increase of T-AOC and SOD activity and the decrease of MDA content in brain tissue.
作者
张玥
陈林
谢春
杨小雪
罗俊
罗喜荣
ZHANG Yue;CHEN Lin;XIE Chun;YANG Xiaoxue;LUO Jun;LUO Xirong(High Efficacy Application of Natural Medicinal Resources Engineering Center of Guizhou&Key Laboratory of Optimal Utilization of Natural Medicine Resources,Guizhou Medical University,Guiyang 550025,Guizhou,China;Department of Pharmacology,School of Basic Medical Sciences,Guizhou Medical University,Guiyang 550025,Guizhou,China;Department of Pharmacology,Qiannan Medical College For Nationalities,Duyun 558013,Guizhou,China;Department of Occupational Health and Environmental Hygiene,School of Public Health,Guizhou Medical University,Guiyang 550025,Guizhou,China;Center for Disease Control and Prevention in Southeast Guizhou,Kaili,556000,Guizhou,China;School of Pharmaceutical Sciences,Guizhou Medical University,Guiyang 550025,Guizhou,China)
出处
《贵州医科大学学报》
CAS
2020年第3期277-280,共4页
Journal of Guizhou Medical University
基金
贵州省科学技术基金[黔科合J字(2012)2028]。
关键词
灵芝属
砷中毒
丙二醛
超氧化物歧化酶
灵芝三萜类化合物
总抗氧化能力
Ganoderma lucidum
arsenic poisoning
malondialdehyde
superoxide dismutase
Ganoderma lucidum triterpenoids(GLT)
total-antioxidant capacity
