摘要
建立超高效液相色谱-串联质谱法测定猪油中磺胺类药物残留的方法。试样经正己烷溶解,加入80%乙腈水溶液提取,经PRIME HLB小柱净化后,供超高效液相色谱-串联质谱检测。用甲醇-0.1%甲酸+2 mol/L甲酸铵水溶液为流动相,经质谱多反应监测(MRM)模式检测。结果显示:18种磺胺类药物均有良好的线性关系,相关系数均大于0.99,检出限为0.5μg/mL,回收率在90.1%~100.0%,RSD在0.17%~5.94%。
A method for the determination of sulfonamides residues in lard by ultra high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established.The sample was dissolved by hexane and extracted by adding 80%acetonitrile water solution,the extracted samples were purified by HLB column and detected by UPLC-MS/MS.Using methanol-0.1%formic acid+ammonium formate water solution as mobile phase,samples were detected by multiple response monitoring(MRM).Results showed a good linearity(R2>0.99)for all compounds.The detection limit was 0.5μg/mL,the recoveries ranged between 90.1%~100.0%,the RSD was 0.17%~5.94%.
作者
王依婷
查圆圆
WANG Yi-ting;ZHA Yuan-yuan(Shanghai Kemao Grain and Oil Food Quality Inspection Co.Ltd.,Shanghai 200333,China)
出处
《粮食与油脂》
北大核心
2020年第4期88-91,共4页
Cereals & Oils
关键词
超高效液相色谱-串联质谱法
磺胺类药物
猪油
药物残留
ultra-high performance liquid chromatography-tandem mass spectrometry
sulfanilamide
lard
drug residue
