摘要
为探明在稻田养殖模式下,不同饲料对克氏原螯虾(Procambarus clarkii)生长、非特异性免疫酶和体成分的影响,于2018年3—5月,将体重为(2.09±0.20)g、体长为(4.54±0.13)cm的克氏原螯虾虾苗随机分为6组(A1~A6组,每组7100尾),其中向A1~A5组分别投喂5种不同饲料(A1组饲喂玉米、A2组饲喂大豆、A3组饲喂添加了复方黄芪多糖蛋白质含量≥26%的饲料、A4组饲喂蛋白质含量≥26%的饲料、A5组饲喂蛋白质含量≥28%的饲料),A6组不投喂饲料,每组设置一个平行组。养殖周期为60 d,测定该虾的生长性能、非特异性免疫以及体成分等指标。结果显示:1)A3组体重增加率最大,与A5组差异不显著(P>0.05),与其它组差异显著(P<0.05);A3组体长增加率最大,与A4和A5组差异不显著(P>0.05),与其它组差异显著(P<0.05)。2)A2组超氧化物歧化酶(SOD)的活性最高,但与其它组差异不显著(P>0.05);A5组酸性磷酸酶(ACP)的活性最高,与A1组差异不显著(P>0.05),与其它组差异显著(P<0.05);A6组溶菌酶(LSZ)的活性最高,与A1组、A2组和A3组差异不显著(P>0.05),但与A4和A5组溶菌酶(LSZ)的差异显著(P<0.05)。3)含肉率和系水力方面,A1组克氏原螯虾的含肉率最高,与其它组差异显著(P<0.05);A3组系水力最高与A2组、A4组和A6组差异不显著(P>0.05),与A1组和A5组差异显著(P<0.05);A6组的含水率最高与其它组差异显著(P<0.05)。4)粗蛋白含量最高的是A5组,与其它各组差异不显著(P>0.05);A1组粗脂肪含量最高,其次为A5组,两组差异不显著(P>0.05),两组与其它各组差异显著(P<0.05);粗灰分含量最高的是A4组,但各组差异不显著(P>0.05)。在本试验条件下,综合分析不同饲料对克氏原螯虾生长、非特异性免疫酶和体成分的影响,添加了复方黄芪多糖蛋白含量≥26%的饲料优于其它饲料。
The effects of different feeds on growth,non-specific immune enzymes and body composition of Crawfish(Procambarus clarkii)were studied under rice field culture mode.Crawfish seedlings(body weight:2.09±0.20 g,body length:4.54±0.13 cm)were randomly divided into six groups(A1-A6,7100 of Procambarus clarkii each group),and five groups were fed 5 different kinds of feeds(A1 group was raised with corn,A2 group was treated with soybean,A3 group was fed with the feed containing≥26% proteins and astragalus polysaccharides,A4 group was raised with the feed containing≥26% proteins,A5 group was fed with the feed containing≥28% proteins),and group A6 were not fed feed as control.Arepeat was set up for each group.The whole field experiment lasted 60 days.After that,the indexes of growth performance,non-specific immune and body composition of crawfish were determined.The results showed as follow.1)The highest gain ratio of body weight of crawfish was observed in the group A3,and it has no significant differences(P>0.05)with the other groups except the group A5(P<0.05),and the highest gain ratio of body length of crawfish was also found in the group A3,which has a significant difference(P<0.05)with the other groups except the group A4 and A5(P>0.05).2)The activity of superoxide dismutase(SOD)of crawfish in the group A2 was highest than the other groups,however,it has no significant difference with the other groups(P>0.05).The highest activity of acid phosphatase(ACP)in the group A5 was found,and it has no significant difference with the group A1(P>0.05),however,it has a significant difference compared with the other groups(P<0.05).The highest activity of lysozyme(LSZ)was determined in the group A6,and it has no significant difference with A1,A2 and A3 groups.However,all of them have significant differences with A4 and A5 groups(P<0.05).3)The highest muscle content was observed in the group A1,and it has a significant difference with the other groups(P<0.05).The moisture holding capacity of crawfish muscle in the group A3 was highest than that in the other groups,and it has no significant difference with A2,A4 and A6 groups(P>0.05),but all of them have significant differences with group A1 and group A5(P<0.05).4)The highest moisture content in muscle was found in group A6,and it has a significant difference with the other groups(P<0.05).The highest crude protein content was determined in the group A5,and it has no significant difference with the other groups(P>0.05).The highest crude fat content was detected in the group A1,and it has no significant difference with the group A5(P>0.05),however,both of them have significant differences with the other groups(P<0.05).The highest crude ash content was founded in group A4,but there was no significant differences(P>0.05)between groups.In this study,the feed containing≥26%proteins and adding astragalus polysaccharides is better than the other feeds based on the analysis of growth performance,non-specific immune enzymes and body composition of crawfish.
作者
刘永涛
董靖
夏京津
王桢月
胥宁
杨秋红
杨移斌
艾晓辉
LIU Yongtao;DONG Jing;XIA Jingjin;WANG Zhenyue;XU ning;YANG Qiuhong;YANG Yibin;AI Xiaohui(Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China;Key Laboratory of Control of Quality and Safety for Aquatic Products,Ministry of Agriculture and rural affairs,Beijing China;Hubei Freshwater Aquaculture Collaborative Innovation Center,Wuhan 43022,China;College of fisheries,Huazhong Agricultural University,Wuhan 430223,China;College of Fisheries and life Science,Shanghai Ocean University,Shanghai 201306,China)
出处
《中国渔业质量与标准》
2020年第1期43-51,共9页
Chinese Fishery Quality and Standards
基金
湖北省“稻田综合种养产业技术体系”项目
现代农业人才支撑计划项目(2016-2020)。
关键词
克氏原螯虾
稻田养殖
饲料
生长
非特异性免疫
含肉率
系水力
体成分
crawfish
rice field culture
feeds
growth
non-specific immune
muscle content
moisture holding capacity
body composition
