摘要
目的建立以高效液相色谱法(HPLC)同时测定肉桂叶中香豆素、肉桂酸、桂皮醛成分含量的方法。方法色谱柱为GL Intertsil ODS3 C18柱(4.6 nm×250 nm,5μm);流动相为乙睛-0.1%磷酸水梯度洗脱;检测波长为290 nm;柱温为25℃。结果香豆素在0.3452~3.452μg范围内与相应的峰面积有良好的线性关系,肉桂酸在0.138~1.38μg范围内与相应的峰面积有良好的线性关系,桂皮醛在0.2142~2.142μg范围内与相应的峰面积有良好的线性关系,相关系数分别为0.9999、0.9999、0.9990;香豆素平均回收率96.02%,RSD为0.25%;肉桂酸平均回收率96.43%,RSD为2.9%;桂皮醛平均回收率95.81%,RSD为2.6%。结论本方法简便、快捷,结果准确度高,重复性好,可作为肉桂叶中香豆素、肉桂酸和桂皮醛含量测定方法。
Objective To establish an HPLC method for the content determination of coumarin,cinnamic acid cinnamaldehyde and in Folium Cinnamoni.Methods The analytical method was carried out with GL Intertsil ODS3 C18(4.6 nm×250 nm,5μm)columnand.The mobile phase was consisted of acetonitrile-0.1%phosphoric acid solution as a flow rate of 1.0 mL/min.The detection wavelength was 290 nm.The column temperature was 25℃.Results The calibration curve was linear within the range of 0.3452~3.452μg,0.138~1.38μg and 0.2142~2.142μg for coumarin,cinnamic acid and cinnamaldehyde.The correlation coefficient r values were respectively 0.9999,0.9999 and 0.9990.The average recoveries were 96.02%(RSD=0.25%),96.43%(RSD=2.9%)and 95.81%(RSD=2.6%)for coumarin,cinnamic acid and cinnamaldehyde.Conclusion The method is simple,accurate,reproducible and precise,and applicable for the determination of coumarin,cinnamaldehyde and cinnamic acid in Folium Cinnamoni.
作者
李耀华
魏江存
梁建丽
黄琪喜
黄瑞松
雷沛霖
LI Yaohua;WEI Jiangcun;LIANG Jianli;HUANG Qixi;HUANG Ruisong;LEI Peilin(Guangxi University of Chinese Medicine,Nanning 530200,Guanxi,China;Guangxi International Zhuang Medical Hospital,Nanning 530201,Guangxi,China;Guangxi Academy of Minority Nationality Medicine and Pharmacology,Nanning 530001,Guangxi,China)
出处
《中华中医药学刊》
CAS
北大核心
2020年第2期54-57,共4页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金(81660701)
国家中医药管理局科研三级实验室中(壮)药化学与质量分析实验室项目(国中医药发2009[21])
广西教育厅项目(201106LX272)
广西高校中药提取纯化与质量分析重点实验室项目(桂教科研[2014]6)
广西中医药大学自然科学研究项目(P2010018)。
