摘要
AP2基因作为一种调控植物花发育的功能基因,参与花分生特性建立。利用生物信息学方法对阿拉伯岩芥AP2蛋白的理化性质、高级结构和系统进化关系等进行了详细的预测和分析。结果表明,除了成员AA40G00375、AA54G00162为稳定蛋白,其余AP2蛋白均为不稳定蛋白,且大多数蛋白定位在细胞核。只有成员AA8G00350属于分泌蛋白,其余均为非分泌蛋白,且均不属于跨膜蛋白。磷酸化位点分析表明AP2蛋白的生物功能主要是通过在丝氨酸(Ser)位点进行磷酸化来实现的。AP2蛋白的二级结构以无规则卷曲为主,各个成员的二级结构元件组成含量大小排列顺序为无规则卷曲>α-螺旋>延伸链>β-转角。其中14个AP2蛋白成员有2个保守结构域,其余均有1个保守结构域。系统进化树表明阿拉伯岩芥AP2基因家族与拟南芥、小麦有较近的亲缘关系。这些结果可为进一步研究其生物学功能提供一定的参考依据。
AP2 gene,as a functional gene regulating plant flower development,is involved in the establishment of floral meristems.The physicochemical properties,advanced structure and phylogenetic relationship of AP2 proteins from Aethionema arabicum were predicted and analyzed in detail by bioinformatics.The study shows that apart from the stable proteins AA40G00375 and AA54G00162,the other AP2 proteins are unstable,and most of them were located in the nuclear.Only member AA8G00350 belongs to secretory proteins,the rest are non-secretory proteins,and they are not transmembrane proteins.Analysis of phosphorylation sites shows that the biological function of AP2 protein is mainly achieved by phosphorylation of serine(Ser)site.The secondary structure of AP2 protein is dominated by random coil,and the content of secondary structural elements of each member is presented in the order of random coil>alpha helix>extended strand>beta turn.Fourteen members of AP2 protein have two conserved domains,and the rest have one conserved domain.Phylogenetic tree shows that AP2 gene family of A.arabicum is closely related to that of Arabidopsis thaliana,Triticum urartu.These results provides some references for further study of its biological function.
作者
付春
刘晓伟
王玲
江纳
杨瑶君
FU Chun;LIU Xiao-wei;WANG Ling(Sichuan Key Laboratory of Bamboo Disease and Pests Control and Resources Development,Leshan Normal University,Leshan,Sichuan 614000;College of Life Science,Leshan Normal University,Leshan,Sichuan 614000)
出处
《安徽农业科学》
CAS
2020年第8期114-123,共10页
Journal of Anhui Agricultural Sciences
基金
四川省科技厅项目(17ZZ019)
乐山师范学院人才启动项目(XJR17005)。
