摘要
目的研究白藜芦醇对小细胞肺癌C-myc基因表达的影响,探讨其在小细胞肺癌中的可能抑癌机制。方法以人小细胞肺癌H446细胞系为研究对象,采取Western blot法和Real-Time PCR法检测不同浓度白藜芦醇及白藜芦醇作用不同时间后C-myc的表达变化,分别以NAC和顺铂为干预进行验证。结果Western blot和Real-time PCR检测结果显示白藜芦醇可调控人小细胞肺癌H446细胞C-myc的表达,并随着作用时间的延长和作用浓度的增加,C-myc的表达进一步下降,具有时间依赖和浓度依赖的特点,差异有统计学意义(P<0.05)。给予NAC干预后,可抑制白藜芦醇对C-myc蛋白表达的调控影响,呈现拮抗作用,差异均有统计学意义(P<0.05)。顺铂可抑制C-myc的表达,与白藜芦醇联用对C-myc调控具有协同作用,Western blot检测结果显示在胞浆蛋白中差异有统计学意义(P<0.05),但在胞核蛋白中,与白藜芦醇组相比,白藜芦醇与顺铂联用,差异无统计学意义(P>0.05)。Real-time PCR检测结果显示顺铂可使C-myc表达水平下调,与白藜芦醇联用具有协同作用,差异有统计学意义(P<0.05)。结论白藜芦醇对小细胞肺癌H446细胞的C-myc表达具有明显的影响,间接说明白藜芦醇对小细胞肺癌中的抑癌作用可能与myc基因有关。
Objective The aim of the present study was to evaluate the effects of resveratrol on C-myc gene expression in small-cell lung cancer(SCLC)cell.Methods The H446 cell line of human small-cell lung cancer was used as the object of this study.The expression levels of C-myc were measured by western blot analysis and Real-time PCR in H446 cells treated with various concentrations of Res for 24 h and treated with the same concentration Res at different times.Cisplatin was used as the intervention for effect verification.Results The measure results of Western blot and Real-time PCR show that resveratrol has regulating effect on the expression of C-myc in H446 cells of human small-cell lung cancer.Along with the extension of the action time and the increase of the concentration,the expression of c-myc further decreased.These effects have the characteristic of concentration-and time-dependence,the difference has statistically significant(P<0.05).In addition,combined treatment with NAC and Res is found to be a inhibiting effect compared with either monotherapy while cisplatin and Res is found to be markedly more effective compared with either monotherapy,the difference has statistically significant(P<0.05).However,the result of Western blot shows that the expression of C-myc in the cytonuclear protein has no significant difference in the combination group and the resveratrol group(P>0.05).Conclusion The present study demonstrated that resveratrol has a significant effect on C-myc expression in H446 cells.These findings suggest that the inhibiting effect of resveratrol on small cell lung cancer may be related to myc gene.
作者
李王平
马李杰
潘蕾
金发光
Li Wangping;Ma Lijie;Pan Lei;Jin Faguang(Department of pulmonary and critical care medicaine, Tangdu Hospital, The Air Force Medical university, Xi′an 710038, China)
出处
《中华肺部疾病杂志（电子版）》
CAS
2020年第2期148-153,共6页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
国家自然科学基金资助项目(81071933)。
