摘要
为了探讨山药对虹鳟(Oncorhynchus mykiss)生长和非特异性免疫功能的影响,将虹鳟分成基础饲料组(对照组)和0.1%、0.2%、0.4%山药提取物组,饲养56 d,进行生长及血清免疫相关指标的检测。结果显示,与对照组相比,0.1%、0.2%、0.4%山药提取物组虹鳟生长性能指标无显著差异,而血清中尿素氮(BUN)含量分别显著降低了51.20%、64.06%和49.80%,补体4(C4)含量分别显著升高了154.35%、144.03%和304.68%,溶菌酶(LZM)活性分别显著升高了42.41%、34.95%和27.89%;0.2%和0.4%山药提取物组总蛋白(TP)含量分别显著升高了36.49%和45.97%,白介素(IL)-6显著升高了45.01%和56.25%,肿瘤坏死因子(TNF-α)显著升高了247.32%和414.15%,超氧化物歧化酶(SOD)活性显著升高了82.14%和110.71%,而碱性磷酸酶(ALP)活性显著降低了51.60%和56.75%;0.1%和0.2%山药提取物组谷丙转氨酶(GPT)活性分别显著降低了77.37%和60.71%;0.1%山药提取物组肌酐(CREA)含量显著降低了41.96%;0.2%山药提取物组IL-2含量显著升高了87.59%;0.4%山药提取物组白蛋白(ALB)含量和酸性磷酸酶(ACP)活性分别显著升高了30.73%和63.77%。上述结果表明,在虹鳟基础饲料中添加山药提取物,对虹鳟生长性能无显著影响,但可提高虹鳟非特异性免疫功能,建议添加量为0.2%~0.4%。
To investigate effects of yam on growth performance and immunity function of rainbow trout(Oncorhynchus mykiss),four test groups were used,which consisted of basic feed group(control),0.1%,0.2%and 0.4%yam extract addition groups.Rainbow trout were randomly allotted to four groups and were fed for 56 d.The results were as follows:compared with the control group,0.1%,0.2%and 0.4%yam extract had no significant effect on the growth performance,but significantly decreased the content of blood urea nitrogen(BUN)and the inhibitory effects were 51.20%,64.06%and 49.80%,respectively,while significantly increased complement 4(C4)level(the induction effects were 154.35%,144.03%and 304.68%,respectively)and lysozyme(LZM)activities(the induction effects were 42.41%,34.95%and 27.89%,respectively)in the serum of rainbow trout;total protein(TP),interleukin-6(IL-6),tumor necrosis factorα(TNF-α),and superoxide dismutase(SOD)levels were significantly increased in the serum of 0.2%and 0.4%groups(the induction effects were 36.49%and 45.97%,45.01%and 56.25%,247.32%and 414.15%,82.14%and 110.71%,respectively),while alkaline phosphatase(ALP)activities in 0.2%and 0.4%groups,glutamic-pyruvic transaminase(GPT)activities in 0.1%and 0.2%groups,CREA content in 0.1%group were significantly decreased(the inhibitory effects were 51.60%and 56.75%,77.37%and 60.71%,41.96%,respectively);interleukin-2(IL-2)content in 0.2%group,albumin(ALB)content and acid phosphatase(ACP)activity in 0.4%group were significantly increased(the induction effects were 87.59%,30.73%and 63.77%,respectively).The above results showed that the growth performance of rainbow trout could not be affected by adding the extracts of yam to the basic feed,while the non-specific immune function could be improved.The recommended dosage was 0.2%—0.4%.
作者
王凡
李聪歌
马雅雯
刘飞
孟祥杰
WANG Fan;LI Congge;MA Yawen;LIU Fei;MENG Xiangjie(College of Life Sciences,Luoyang Normal University,Luoyang 471934,China)
出处
《河南农业科学》
北大核心
2020年第4期167-172,共6页
Journal of Henan Agricultural Sciences
基金
河南省科技攻关项目(182102110407,172102110104)
洛阳师范学院应用科学与技术研究基金项目(2016-YYJJ-02)。
关键词
虹鳟
山药提取物
生长
非特异性免疫
血清
Rainbow trout
Yam extract
Growth
Non-specific immunity
Serum
