摘要
目的探索Morris水迷宫的定位航行实验在连续筛选急性CO中毒迟发性脑病(DEACMP)模型中的应用价值。方法选择成年雄性SD大鼠,经过Morris水迷宫的定位航行试验训练,剔除60 s以上找到平台的SD大鼠,筛选合格SD大鼠108只,随机分为DEACMP组(腹腔注射纯品CO气体)、空气组(腹腔注射空气)、空白组(不进行任何预处理),每组36只。将三组各分成6个亚组(造模前、造模后第1天、第7天、第14天、第21天、第28天),每个亚组6只。从首次造模后2 h开始至24 h监测动脉碳氧血红蛋白(HbCO)浓度,在6个筛选时点上行Morris水迷宫定位航行试验,检测DEACMP大鼠认知功能,筛选出DEACMP模型,同时制备大鼠脑组织石蜡切片,观察海马CA3区变性坏死的神经元。结果(1)DEACMP组的平均潜伏期与空白组、空气组比较,在造模前、造模后第1天、第7天差异均无统计学意义(P均>0.05),而在第14天、第21天、第28天均长于空白组、空气组(P均<0.05)。(2)HE染色观察海马CA3区神经元变性坏死,将这种变性坏死神经元定为阳性细胞。DEACMP组的阳性细胞计数与空白组、空气组比较,在造模前、造模后第1天、第7天差异均无统计学意义(P均>0.05),而在第14天、第21天、第28天多于空白组、空气组(P均<0.05)。(3)Pearson相关性分析,DEACMP组染毒前后不同时间段的平均潜伏期与阳性细胞数之间呈正相关(r=0.915,P<0.001)。结论Morris水迷宫的定位航行实验是一种简易的检测DEACMP大鼠认知功能的可靠方法。
Objective To explore the application value of the Morris water maze positioning navigation experiment in the continuous screening of acute carbon monoxide poisoning delayed encephalopathy(DEACMP)model.Methods Adult male SD rats were selected.After Morris water maze positioning and navigation test training,those who could not find a platform for more than 60 s were excluded.Then 108 qualified SD rats were selected and randomly divided into DEACMP group,air group and blank group,and intraperitoneal injection was given.Pure CO gas,intraperitoneal injection of air,and intraperitoneal cavity were not interfered,36 animals in each group.Based on the 6 time points before and after modeling(before modeling,1st day,7th day,14th day,21st day,and 28th day),the DEACMP group,air group,and blank group were divided into 6 subgroups,with 6 in each subgroup.From 2 h to 24 h after the first intervention,the three groups monitored the arterial oxyhemoglobin(HbCO)concentration,and then performed the Morris water maze positioning sailing test at six screening points to test the cognitive function of DEACMP rats and screen out the DEACMP model.Paraffin sections of rats’brain tissue were prepared,and the neurons in the hippocampal CA3 area were observed for degeneration and necrosis.Results(1)There was no significant difference between the average latency of Morris water maze in the DEACMP group and the blank group and the air group before modeling,1st day,and 7th day(P all>0.05),but DEACMP group were longer at 14th day,21st day,28th day than the blank group and the air group(P all<0.05).(2)HE staining was used to observe degeneration and necrosis of neurons in the CA3 area of the hippocampus,and the degenerative and necrotic neurons were identified as positive cells.There was no significant difference between the positive cell count of the DEACMP group and the blank group and the air group before the modeling,1st day,and 7th day(P all>0.05),but DEACMP group were more at 14th day,21st day,and 28th day than the blank group and the air group(P all<0.05).(3)Pearson correlation analysis showed that there was a positive correlation between the average incubation period and the number of positive cells in different time periods before and after exposure to DEACMP(r=0.915,P<0.001).Conclusion Morris water maze navigation experiment is a simple and reliable method to detect the cognitive function of DEACMP rats.
作者
胡小东
关浩
杨立山
HU Xiaodong;GUAN Hao;YANG Lishan(Ningxia Medical University,Yinchuan750004,China;Department of Emergency,the General Hospital of Ningxia Medical University,Yinchuan750004,China)
出处
《宁夏医科大学学报》
2020年第1期25-29,共5页
Journal of Ningxia Medical University
基金
宁夏自然科学基金(NZ17162)。
