摘要
目的研究新型冠状病毒(2019-nCoV)实验室核酸检测的原理方法,探讨实时荧光定量PCR方法在新型冠状病毒实验室检测中的价值与实验室注意事项。方法采用实时荧光定量PCR方法检测2020年1月23日-2020年2月2日就诊于解放军总医院海南医院发热门诊的患者以及筛查的湖北旅居者的咽拭子,采用实时荧光定量PCR的方法检测新型冠状病毒开放读码框1ab(ORF1ab)、核壳蛋白(N)基因。结果共检测的3824例标本中,阳性病例35例,阳性率0.91%。结论疫情期间对发热门诊患者及湖北旅居者进行2019-nCoV核酸筛查是非常有必要的,实时荧光定量PCR是一种检测2019-nCoV科学可靠的方法,能快速准确地检测待检标本中的2019-nCoV核酸,对监测和控制疫情有重要意义。
OBJECTIVE To study the principles of detection of nucleic acid of 2019-nCoV in medical laboratory, explore the value of real-time fluorescence quantitative PCR in laboratory detection of 2019-nCoV, and understand the matters that need the attention. METHODS From Jan. 23, 2020 to Feb. 2, 2020 the throat swab specimens of the patients of fever clinic and the screened people who had the history of travelling or living in Hubei Province were detected in Hainan Hospital, PLA General Hospital by using real-time fluorescence quantitative PCR, and the two genes, 2019-nCoV open reading frame 1 ab(ORF1 ab) and nucleoprotein(N) gene, were detected with the use of real-time fluorescence quantitative PCR. RESULTS Of totally 3 824 specimens, 35 were positive, with the positive rate 0.91%. CONCLUSION It is very necessary to conduct the screening test of nucleic acid of 2019-nCoV for the patients of fever clinic and the people who have the history of travelling or living in Hubei Province during the epidemic, the real-time fluorescence quantitative PCR is a scientific and reliable approach for detection of 2019-nCoV, it can detect the nucleic acid rapidly and accurately and has great significance in monitoring and control of the epidemic.
作者
匡慧慧
于梅
于帅
周汉王
王依函
朱乔
肖征
李慧灵
KUANG Hui-hui;YU Mei;YU Shuai;ZHOU Han-wang;WANG Yi-han;ZHU Qiao;XIAO Zheng;LI Hui-ling(Hainan Hospital,PLA General Hospital,Hainan 572000,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2020年第6期830-833,共4页
Chinese Journal of Nosocomiology
