摘要
【目的】研究茶叶儿茶素HPLC指纹图谱与其自由基清除活性的关系。【方法】采用70%甲醇对绿茶、白茶、闽北乌龙、闽南乌龙、红茶5类共25批次样本进行提取;以福林酚法测定总酚含量;以1,1-二苯基-2-三硝基苯肼(DPPH)法研究自由基清除活性;同时通过高效液相色谱法(HPLC)获取指纹图谱数据并对所获取的指纹图谱数据进行主成分分析;采用偏最小二乘回归分析研究HPLC指纹图谱与DPPH清除活性的谱效关系,结合皮尔逊(Pearson)相关性分析对偏最小二乘回归模型进行验证。【结果】获取25批次样本的HPLC指纹图谱,确定了8个儿茶素类化合物,成功构建了偏最小二乘回归方程,其决定系数R2=0.9009。结果表明表没食子儿茶素没食子酸酯(EGCG)、表没食子儿茶素(EGC)、表儿茶素没食子酸酯(ECG)与DPPH清除力相关性最强,均呈极显著正相关;没食子酸(GA)与DPPH清除力呈负相关。Pearson相关性分析结果与偏最小二乘回归模型结果基本一致。【结论】本研究通过HPLC指纹图谱信息可预测抗氧化活性。
【Objective】Correlation between the high-performance liquid chromatography(HPLC)fingerprints and the DPPH radical scavenging activities of catechins from varieties of teas was studied.【Method】From 5 categories of teas including green tea,white tea,northern Fujian oolong,southern Fujian oolong,and black tea,25 specimens were collected and extracted with 70%methanol.The extracts were used to determine polyphenols content by the Folin-Ciocalteu method,the free radical scavenging activity by[1,1-diphenyl-2-picrylhydrazylradical2,2-Diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl,DPPH]assay,and the fingerprints by HPLC for a principal component analysis.A correlation model between the HPLC fingerprints and DPPH radical scavenging activity was established by a partial least squares regression analysis and validated by the Pearson analysis.【Result】The HPLC fingerprints of the 25 specimens had 8 common compounds.The established regression model showed a coefficient of determination of R2=0.9009.The 3 compounds,epigallocatechin gallate(EGCG),epigallocatechin(EGC),and epicatechin gallate(ECG),exhibited the strongest and significant correlations with DPPH radical scavenging activity.On the other hand,gallic acid(GA)correlated inversely with the scavenging activity.The Pearson analysis showed a result consistent with what the partial least squares regression test did.【Conclusion】It appeared that the antioxidant activity of catechins in the various teas could be satisfactorily predicted by HPLC fingerprints.
作者
林清霞
王丽丽
杨军国
宋振硕
陈林
LIN Qing-xia;WANG Li-li;YANG Jun-guo;SONG Zhen-shuo;CHEN Lin(Tea Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;School of Biological Science and Biotechnology,Minnan Normal University,Zhangzhou,Fujian 363000,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2020年第2期210-216,共7页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划公益类专项(2019R1029-3)
福建省农业科学院青年人才创新基金项目(YC2018-7)。
关键词
儿茶素
抗氧化
高效液相色谱(HPLC)
谱效关系
catechins
antioxidant activities
high-performance liquid chromatography(HPLC)
spectrum-effect relationship
