摘要
建立新疆一枝蒿药材双波长HPLC指纹图谱的测定方法,为药材质量标准提升提供科学依据。采用HPLC法,Zorbax SB-C18色谱柱(4.6 mm×250 mm,5μm),柱温32℃;流动相:甲醇-0.2%甲酸水梯度洗脱;流速1.0 mL/min;检测器:DAD检测波长245和325 nm;进样量20μL。建立了新疆一枝蒿的HPLC指纹图谱,分别在245和325 nm处确定了7个和8个共有峰,并确证了槲皮素、绿原酸、木犀草素、芹菜素、蔓荆子素5个色谱峰,9批次新疆一枝蒿药材与生成对照指纹图谱的相似度大于0.9。首次建立了新疆一枝蒿药材双波长HPLC指纹图谱,所建立的指纹图谱测定方法精密度、稳定性和重复性良好,可用于新疆一枝蒿药材的鉴别和质量控制。
To establish HPLC dual-wavelength fingerprint of Artemisia rupestris L.,and provide a scientific basis for the improvement of its quality specifications.The separation was performed on an Agilent Zorbax SB-C18(4.6 mm×250 mm,5μm)column maintained at 32℃,with methanol-0.2%formic acid-water gradient elution at flow rate of 1.0 mL/min and the UV detection wavelength at both 245 and 325 nm.The sample injection volume was 20μL.The fingerprint of Artemisia rupestris L.was established.7 and 8 common peaks were found,respectively,of which 5 common peaks were identified,and the similarity among 9 batches of Artemisia rupestris L.and the fingerprints of control was over 0.9.The HPLC dual-wavelength fingerprint of Artemisia rupestris L.was established for the first time,providing a new scientific basis for its identification and quality control.
作者
张逸凡
鲍凤霞
夏振江
尚靖
ZHANG Yifan;BAO Fengxia;XIA Zhenjiang;SHANG Jing(School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198;State Key Laboratory of Pollution Control and Resources Reuse,School of the Environment,Nanjing University,Nanjing 210023,China)
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2020年第2期175-179,共5页
Journal of China Pharmaceutical University
基金
国家自然科学基金青年科学基金项目(No.41907365)
青海省藏药药理学和安全性评价研究重点实验室开放课题基金(No.2019-ZY-02)。
关键词
新疆一枝蒿
HPLC
双波长
指纹图谱
质量控制
Artemisia rupestris L.
HPLC
dual-wavelength
fingerprint
quality control
