摘要
目的建立高效液相色谱法(HPLC)对半夏及其伪品进行指纹图谱分析,以鉴定半夏的真伪。方法用SB-Aq色谱柱(4.6 mm×250 mm,5μm),以0.2%磷酸二氢铵溶液-甲醇为流动相进行梯度洗脱,进样量20μL,流速0.8 mL/min,柱温30℃,采用双波长检测。结果生半夏共有10个共有峰,通过对照品比对指认了其中5个共有峰;伪品半夏中同时含有8种有效成分;随机选取部分生半夏与伪品半夏样品建立叠加指纹图谱得出:虎掌南星于34~38 min出现2个特征峰;姜水半夏在32~34 min出现2个特征峰;而水半夏出峰整体峰高较低,峰面积偏小。结论建立的指纹图谱特征性强,能明显区分半夏与其伪品水半夏及虎掌南星,可用于半夏的鉴别和质量控制。
OBJECTIVE To develop a high performance liquid chromatography(HPLC)method for the fingerprint analysis of Pinellia ternata and its authenticity identification.METHODS HPLC analysis was performed on a SB-Aq column(4.6 mm×250 mm,5μm)with the mixture of 0.2%ammonium dihydrogen phosphate solution(pH=3.0)(A)and methanol(B)as mobile phase in gradient mode.The column temperature was set at 30℃and dual wavelength detection was used.RESULTS The HPLC fingerprint of Pinellia ternata,showing 10 characteristic peaks,was established from raw Pinellia ternata,and 5 were identified by standard comparison,and 8 common constituents were identified in fake Pinellia ternata samples.An overlay fingerprint were established with samples randomly selected:From 34 to 38 min,there were two characteristic peaks in the Pedate Rhizome Pinellia,and two appeared at 32 to 34 min in the Rhizoma Typhonii Flagelliformis prepared with ginger juice,while the peak height in the raw Typhonii flagelliformis Rhizoma was lower and the peak area was smaller.CONCLUSION The fingerprint of Pinellia ternata is significantly different from its confused herbs,which can be applied to the identification and quality control of Pinellia ternata.
作者
王珏
郭钰昕
姚毅
张剑
李津
朱育凤
刘史佳
WANG Jue;GUO Yu-xin;YAO Yi;ZHANG Jian;LI Jin;ZHU Yu-feng;LIU Shi-jia(Jiangsu Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, 210029, China;School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing, 210009, China;School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023, China)
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2020年第2期267-272,共6页
Journal of Nanjing University of Traditional Chinese Medicine
基金
国家自然科学基金(81774096,81873309)
江苏省自然科学基金(BK20161610)
江苏省“六大人才高峰”高层次人才项目(WSN-051)
江苏省青年医学人才(QNRC2016642)
中华中医药学会2017-2019年度青年人才托举工程项目(QNRC2-B04)
高层次卫生人才项目(LGY2018061)
江苏省中医院科技项目(Y18010,Y2018RC32)
江苏省中医药局科技项目(YB2017025)。
关键词
半夏
指纹图谱
伪品
鉴别
质量控制
Pinellia ternata
fingerprints
counterfeit
identification
quality control
