摘要
该研究将实时荧光定量PCR技术(real-time fluorescence quantitative PCR,rPCR)与微滴式数字PCR技术(micro-droplet digital PCR,ddPCR)相结合,利用ddPCR建立拷贝数和羊肉质量的函数关系,确立了基于rPCR定量检测羊肉含量的方法。特异性实验结果表明,除绵羊和山羊外,非目标物种DNA未出现特异性扩增;灵敏度实验结果表明,该方法的最低检出限为0.01 ng/μL;通过对已知成分的混合样品和市售样品的检测表明,该方法能够对含量在5%以上的羊肉进行准确定量。因此,该方法在肉制品中羊肉含量检测和掺假鉴别方面具有较好应用潜力。
In order to accurately quantify the content of mutton in meat products,this study established the functional relationship between copy number and mutton weight by using micro-droplet digital PCR(ddPCR).And a quantitative detection method of mutton content based on real-time fluorescence quantitative PCR(rPCR)were also developed.The result of specific experiment showed that the DNA of sheep and goat could be specifically amplified and the detection limit was 0.01 ng/μL.For the mixed samples and the commercial samples,this method could precisely quantify more than 5%of mutton contents in meat products.This method has a potential application in the detection of mutton content in meat products.
作者
金鹭
陈传君
林华
胡滨
韩国全
陈世界
张婧
安微
杨苗
JIN Lu;CHEN Chuanjun;LIN Hua;HU Bin;HAN Guoquan;CHEN Shijie;ZHANG Jing;AN Wei;YANG Miao(College of Food,Sichuan Agriculture University,Ya′an 625000,China;Chengdu Customs,Chengdu 61000,China;Food Safety Detection Key Laboratory of Sichuan,Chengdu 61000,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2020年第8期246-253,共8页
Food and Fermentation Industries
基金
国家海关总署科技计划项目(动物源性成分定量检测技术及在非法添加打假执法中的应用研究(2017IK019))。
关键词
羊肉
实时荧光定量PCR
微滴数字PCR
定量检测
mutton
real-time fluorescence quantitative PCR
droplet digital PCR
quantitative determination
