摘要
目的优化纤维素酶辅助提取藕节多糖工艺,并研究其动力学、热力学。方法在单因素试验基础上,以加酶量、介质p H、酶解温度、酶解时间为影响因素,多糖得率为评价指标,Box-Behnken响应面法优化提取工艺。对提取期间的动力学、热力学进行分析。结果最佳条件为加酶量0.9%,介质p H 5.0,酶解温度47℃,酶解时间2.5 h,多糖得率为5.86%。加入纤维素酶后,多糖得率、提取速率常数(k)、表面扩散系数(Ds)提高。提取过程符合一级动力学模型,吸热熵增加,放热熵减小,为自发过程。结论该方法稳定可靠,可用于纤维素酶辅助提取藕节多糖。
AIM To optimize the cellulase-assisted extraction process for polysaccharides from Nelumbinis Rhizomatis Nodus and to study their kinetics and thermodynamics. METHODS With enzyme consumption,medium pH,enzymolysis temperature and enzymolysis time as influencing factors,polysaccharides yield as an evaluation index,the extraction process was optimized by Box-Behnken response surface method on the basis of single factor test. The kinetics and thermodynamics were analyzed during the extraction. RESULTS The optimal conditions were determined to be 0. 9% for enzyme consumption,5. 0 for medium p H,47 ℃ for enzymolysis temperature,and2. 5 h for enzymolysis time,the polysaccharides yield was 5. 86%. After the addition of cellulase,the polysaccharides yield,extraction rate constant( k) and surface diffusion coefficient( Ds) were elevated. The spontaneous extraction process accorded with first-order kinetic model,along with increased endothermic entropy and decreased exothermic entropy. CONCLUSION This stable and reliable method can be used for the cellulase-assisted extraction for polysaccharides from Nelumbinis Rhizomatis Nodus.
作者
王占一
赵静
朱天顺
胡锦钢
赵春林
向兰
WANG Zhan yi;ZHAO Jing;ZHU Tian shun;HU Jin gang;ZHAO Chun lin;XIANG Lan(College of Food Science and Pharmaceutical Engineering,Zaozhuang University,Zaozhuang 277160,China;College of Pharmacy,Shandong University,Jinan 250012,China)
出处
《中成药》
CAS
CSCD
北大核心
2020年第4期836-842,共7页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(51809228)
中医药公共卫生服务补助专项“全国中药资源普查项目”(财社[2018]43)。
关键词
藕节
多糖
纤维素酶辅助提取
动力学
热力学
Box-Behnken响应面法
Nelumbinis Rhizomatis Nodus
polysaccharides
cellulase-assisted extraction
kinetics
thermodynamics
Box-Behnken response surface method
