摘要
目的分析河北地区呼吸道合胞病毒(respiratory syncytial virus,RSV)引起儿童呼吸系统疾病的流行特征及外周血淋巴细胞亚群和炎性指标的变化。方法收集河北省儿童医院2018年全年因呼吸系统感染入院治疗的患儿痰液标本9491份及配对外周静脉血标本9491份。采用13种呼吸道病原体多重检测试剂筛选出RSV阳性痰液标本,流式细胞术检测随机筛选出的RSV阳性患儿外周血标本中T、B淋巴细胞、NK细胞,Roche E411发光分析仪测定降钙素原(PCT),Roche Cobas 8000 C701生化分析仪测定超敏C反应蛋白(hs-CRP),Sysmex XN-BN3血球仪检测白细胞(WBC)。结果2018年河北地区患呼吸系统疾病儿童中RSV阳性检出率为13.08%,不同年龄段患儿检出率差异有统计学意义(χ^2=479.2976,P<0.0001),RSV感染检出率随年龄增加逐渐降低(χ^2=-20.2827,P<0.0001),男性患儿检出率高于女性患儿(χ^2=34.5527,P<0.0001)。RSV与其他呼吸道病原体的混合感染发生率为29.49%(366/1241),其中与RSV发生混合感染的主要为人鼻病毒(human rhinovirus,HRV),占12.09%(150/1241),其次是腺病毒(adenovirus,ADV),占3.22%(40/1241)。RSV感染的主要流行季节为冬季和春季,单纯感染和混合感染的流行趋势一致。RSV单纯感染和混合感染组间炎性指标、WBC(P<0.01)、CD4+细胞(P=0.015)、CD4+/CD8+细胞(P=0.016)差异有统计学意义。结论RSV是河北地区儿童呼吸系统疾病的常见病原,患者年龄越小越容易被感染。RSV易与HRV、ADV发生混合感染,且河北地区RSV的流行季节为冬季和春季。RSV单纯感染与混合感染可能同时存在免疫功能紊乱情况。
Objective To analyze the epidemiological characteristics of respiratory syncytial virus(RSV)-caused respiratory diseases in children in Hebei Province and the changes in peripheral blood lymphocyte subsets and inflammatory indexes.Methods A total of 9491 sputum specimens and 9491 paired peripheral blood specimens were collected from children admitted to Hebei Children′s Hospital for respiratory infection in 2018.RSV-positive sputum specimens were screened by multiple detection reagents for 13 kinds of respiratory pathogens.Flow cytometry was uses to detect T and B lymphocytes and NK cells in peripheral blood samples of randomly screened RSV-positive children.Procalcitonin(PCT)was measured by Roche E411 luminescence analyzer.Hypersensitive C-reactive protein(hs-CRP)was detected by Roche Cobas 8000 C701 biochemical analyzer.White blood cells(WBC)were measured by Sysmex XN-BN3 hematometer.Results The positive rate of RSV in children with respiratory diseases was 13.08%in Hebei Province in 2018.There were significant differences in RSV-positive rates among different age groups(χ^2=479.2976,P<0.0001).The positive rate of RSV decreased gradually with age(χ^2=-20.2827,P<0.0001)and was higher in male than in female(χ^2=34.5527,P<0.0001).The incidence of co-infection of RSV with other respiratory pathogens was 29.49%(366/1241),mainly caused by human rhinovirus(HRV,150/1241)and adenovirus(ADV,40/1241).The main epidemic seasons of RSV infection were winter and spring.The epidemic trends of simple RSV infection and co-infection were consistent.There were significant differences in inflammatory indexes,WBC(P<0.01),CD4+(P=0.015)and CD4+/CD8+cells(P=0.016)between simple RSV infection and co-infection groups.Conclusions RSV was a common pathogen causing respiratory diseases in children in Hebei Province.The younger the children were,the more likely they were to be infected with RSV.RSV infection was easily complicated by HRV or ADV infection.The epidemic seasons of RSV infection in Hebei were winter and spring.Both simple infection and co-infection of RSV might result in immune dysfunction.
作者
郭巍巍
严小桐
冯志山
黄慧
王乐
李贵霞
吴勇
Guo Weiwei;Yan Xiaotong;Feng Zhishan;Huang Hui;Wang Le;Li Guixia;Wu Yong(Pediatric Research Institute of Hebei Children′s Hospital,Shijiazhuang 050030,China;Hebei Provincial People′s Hospital,Shijiazhuang 050057,China;Laboratory Department of Hebei Children′s Hospital,Shijiazhuang 050030,China;Ningbo Health Gene Technologies Co.,Ltd,Ningbo 315040,China)
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2020年第2期140-145,共6页
Chinese Journal of Microbiology and Immunology
基金
河北省2017年度医学科学研究重点课题计划(20170400)。
关键词
呼吸道合胞病毒
淋巴细胞亚群
分子流行病学
免疫
Respiratory syncytial virus
Lymphocyte subsets
Molecular epidemiology
Immunity
