摘要
目的:观察糖尿病(DM)大鼠中医外观体征和证候相关实验室指标的变化特点,分析糖尿病大鼠证候演变规律,为糖尿病证候模型的建立提供思路。方法:腹腔注射65 mg·kg-1链脲佐菌素建立大鼠糖尿病模型,以72 h(0 w)空腹血糖≥16.7 mmol·L-1作为成模标准。成模第0 w、3 w、4 w、5 w和10 w观察大鼠外观体征:体质量,毛色毛态,精神状态,行为状态,食量,饮水量,二便情况,舌、爪、鼻特定区域红色程度C值和自主活动能力;并测定对应时间点实验室指标:大鼠血浆葡萄糖、FT3、FT4、cAMP、cGMP、皮质醇含量,计算cAMP/cGMP比值。结果:与对照组相比,DM大鼠在成模0 w食量、饮水量和尿量显著升高,持续至10 w(P<0.01),体质量、鼻色红色程度C值、自主活动能力、血浆FT3和FT4浓度显著降低,持续至10 w(P<0.01);成模3 w血浆cAMP/cGMP显著升高,持续至10 w(P<0.05),且出现大便干结呈黑色、毛色枯黄无光泽、毛态竖立症状;成模4 w血浆皮质醇浓度显著升高,持续至10 w(P<0.01);成模5 w DM大鼠均出现大便干稀不调呈黄色和精神萎靡的症状;第10 w舌色红色程度C值显著低于对照组(P<0.01)。结论:以65 mg/kg的剂量腹腔注射链脲佐菌素所建立的糖尿病大鼠10 w内模型稳定;早期以内热偏盛为主,STZ可损伤机体阳气,故兼有轻度阳虚表现,且一直贯穿始终。3~5 w出现气阴两虚的表现,10 w表现为阴阳两虚。
Objective:To observe the characteristics of changes in the appearance,signs of TCM and laboratory indexes related to syndromes in rats with diabetic mellitus(DM),and to analyze the evolution of syndromes in DM rats,thus to provide ideas for the establishment of DM syndrome models.Methods:DM model was established by intraperitoneal injection of streptozotocin(65 mg/kg)in rats,the fasting blood glucose≥16.7 mmol/L at 72 h(0 week)was used as the model standard.At 0 week,3 w,4 w,5 w,and 10 w after modeling,the appearance signs,body weight,hair color and hair state,mental state,behavioral status,food intake,water consumption,urine and stool condition,red degree C value of tongue,claw and nose in specific areas,as well as ability to move independently were observed in the rats.The laboratory indexes at the corresponding time points were determined.The plasma glucose,FT3,FT4,cAMP,cGMP and cortisol content were measured,and cAMP/cGMP was calculated.Results:Compared to those in the control group,food intake,water intake and urine output were significantly increased during model 0w in the DM rats,which continued to 10 w(P<0.01).Body weight,nasal color C,autonomic activity,plasma concentrations of FT3 and FT4 decreased significantly and lasted to 10 w(P<0.01).The plasma cAMP/cGMP increased significantly 3 w after modeling and continued to 10 w(P<0.05).Black dry stool,dull yellow hair,and the bristiling hair could be seen in the rats.Plasma cortisol concentration increased significantly at 4w after modeling,which lasted to 10 w(P<0.01).DM rats at 5 w after modeling showed symptoms of dryness and thinness and yellowness of stool,as well as low spirit.C degree value of tongue redness was significantly lower than the control group at 10 w(P<0.01).Conclusion:The DM model established by intraperitoneal injection of streptozotocin at a dose of 65 mg/kg is stable within 10 weeks.In the early stage,the internal heat is predominant,and STZ can damage the body's yang-qi,so mild yang-deficiency can be seen,which has been throughout the 10 weeks.Deficiency of qi and yin appears in 3-5 weeks,and deficiency yin and yang appears in 10 weeks.
作者
赵瑛
王婕
杨明艳
王冬雪
郇帅
ZHAO Ying;WANG Jie;YANG Mingyan;WANG Dongxue;HUAN Shuai(College of Pharmacy, Harbin University of Commerce, Harbin 150076, China)
出处
《中医药学报》
CAS
2020年第3期11-16,共6页
Acta Chinese Medicine and Pharmacology
基金
国家自然科学基金资助项目(81673871)。
关键词
糖尿病大鼠
中医证候
热证
阴虚
阳虚
Diabetic rats
TCM syndromes
Heat syndrome
Yin deficiency
Yang deficiency
