摘要
目的了解大连市2018年HIV抗体确证试验结果为不确定和阴性的样本随访检测抗体阳转情况,分析HIV抗体筛查、确证结果不符的可能原因。方法对2018年大连市各筛查实验室的HIV筛查试验有反应的样本进行抗体确证试验(蛋白免疫印迹法,western blot,WB),对确证试验结果为不确定和阴性的样本进行随访检测。结果大连市2018年艾滋病筛查试验有反应样本中与确证试验结果不符共计218份,随访后发生HIV抗体阳转16份。HIV抗体确证试验不确定和阴性样本抗体阳转率分别为31.58%(12/38)和2.22%(4/180)(P<0.01)。化学发光、ELISA和金标快速筛查试验有反应样本抗体阳转率分别为7.69%(13/169),4.00%(1/25)和4.35%(1/23)(P>0.05)。化学发光试验S/CO值>15的样本阳转率(60.00%,9/15)高于S/CO值<15的样本(2.59%,4/154)(P<0.01)。结论 HIV抗体确证试验阴性和不确定样本可能为早期感染。
Objective To understand the HIV antibody positive seroconversion of the samples in which HIV screening tests were inconsistent with antibody confirmatory test results in Dalian city in 2018,and understand the possible reasons for the inconsistent results of HIV serological antibody screening and confirmatory test. Methods The HIV antibody confirmatory test(Western blot,WB) was performed on HIV screening positive samples from all screening laboratorys in Dalian city in 2018,then tracked and tested samples with confirmatory test results that were uncertain and negative. Results A total of 218 samples in which the screening results of the test were inconsistent with the results of the confirmatory test were found in 2018 in Dalian city,16 cases showed HIV antibody positive seroconversion after follow-up.The HIV positive seroconversion rate of the uncertain and negative samples of the initial HIV antibody confirmatory test was 31.58%(12/38) and 2.22%(4/180) respectively(P<0.01).In the chemiluminescence,ELISA and gold standard rapid screening tests,HIV positive seroconversion rate was 7.69%(13/169),4.00%(1/25) and 4.35%(1/23)(P>0.05;P>0.05),respectively.HIV positive seroconversion rate(60.00%,9/15) with S/CO value >15 was significantly higher than that with S/CO value <15(2.59%,4/154)(P<0.01). Conclusion HIV antibody confirmatory test negative and uncertain samples may be caused by early infection.
作者
刘大鹏
吕秋月
孙茂利
纪颖
张宁
赵志杰
李程
刘颖丽
LIU Da-peng;LV Qiu-yue;SUN Mao-li;JI Ying;ZHANG Ning;ZHAO Zhi-jie;LI Cheng;LIU Ying-li(Dalian City Center for Disease Control und Prevention,Dalian,Liaoning,116021,China)
出处
《预防医学论坛》
2020年第2期89-92,共4页
Preventive Medicine Tribune
基金
大连市医学科学研究计划项目(1511079)。
关键词
艾滋病
抗体
确证试验
抗体阳转
随访
AIDS
Antibody
Confirmatory test
Positive seroconversion
Follow-up
