摘要
目的探讨血清S100B蛋白、神经元特异性烯醇化酶(NSE)与新生儿缺氧缺血性脑病(HIE)的相关性。方法选取68例HIE患儿,根据病情分为重度HIE组(20例)、中度HIE组(25例)和轻度HIE组(23例)。以25名健康新生儿作为正常对照组。检测所有对象血清S100B蛋白、NSE水平,并进行新生儿神经行为(NBNA)评分,计算NBNA评分异常率。动态观察HIE患儿出生后24 h、48 h、72 h、7 d的血清NSE及S100B蛋白的变化。结果正常对照组、轻度HIE组、中度HIE组、重度HIE组S100B蛋白、NSE水平及NBNA评分异常率依次升高,NBNA评分依次降低,各组间差异均有统计学意义(P<0.001)。血清S100蛋白、NSE水平与NBNA评分呈负相关(r值分别为-0.243、-0.542,P<0.001),与HIE严重程度呈正相关(r值分别为0.398、0.476,P<0.001)。随着出生时间的延长,重度HIE组、中度HIE组、轻度HIE组血清S100B蛋白和NSE水平均逐渐降低(P<0.001)。轻度HIE组、中度HIE组、重度HIE组同一时间点血清S100B蛋白和NSE水平均依次升高(P<0.001)。结论血清S100B蛋白、NSE水平与HIE病情严重程度、患儿神经行为有关,或可用于HIE的早期诊断和预后评估。
Objective To investigate the correlation between neonatal hypoxic-ischemic encephalopathy(HIE)and neurobehavior with S100B protein and neuron-specific enolase(NSE).Methods A total of 68 children with HIE were enrolled.They were classified into severe HIE group(20 cases),moderate HIE group(25 cases)and mild HIE group(23 cases).Totally,25 healthy newborns were enrolled as healthy control group.Serum S100B protein and NSE levels,neonatal behavioral neurological assessment(NBNA)score and NBNA score abnormality rate were determined.The changes of serum NSE and S100B protein in HIE group at 24 h,48 h,72 h and 7 d after birth were observed dynamically.Results In healthy control group,mild HIE group,moderate HIE group,severe HIE group,S100B protein and NSE levels and NBNA score abnormality rate were successively increased,and NBNA score was successively decreased(P<0.001).Serum S100B protein and NSE levels were negatively correlated with NBNA score(r=-0.243 and-0.542,respectively,P<0.001),and they were positively correlated with HIE severity(r=0.398 and 0.476,respectively,P<0.001).With the extension of birth time,serum S100B protein and NSE levels in severe HIE group,moderate HIE group and mild HIE group were gradually decreased(P<0.001).Serum S100B protein and NSE levels in mild HIE group,moderate HIE group and severe HIE group were successively increased at the same time point(P<0.001).Conclusions Serum S100B protein and NSE levels are correlated with the severity of HIE and the neurobehavior of children,which can be used for the early diagnosis and prognosis of HIE.
作者
付慧
熊虹
康文清
许邦礼
卢瑞存
FU Hui;XIONG Hong;KANG Wenqing;XU Bangli;LU Ruicun(Department of Neonatology,the Children's Hospital of Henan Province,Zhengzhou 450003,Henan,China)
出处
《检验医学》
CAS
2020年第4期300-303,共4页
Laboratory Medicine
