摘要
目的:探究榄香烯对人NSCLC细胞系A549细胞增殖、周期和凋亡的影响,确定其最佳用药浓度以及时间。方法:将人NSCLC细胞系A549细胞进行体外培养,分析榄香烯作用;采用Cell Counting Kit-8法检测榄香烯对细胞的增殖作用;采用流式细胞术检测细胞周期和凋亡。结果:细胞增殖抑制率随榄香烯浓度的升高,呈增高趋势。细胞周期检测及分析结果显示,经榄香烯作用48h和72h后,进入G2/M期的细胞明显减少,而sub-G1期细胞显著增多。随药物浓度增长,细胞凋亡率显著提升。结论:β-榄香烯可明显抑制非小细胞肺癌A549细胞的增殖,阻断细胞周期,促进细胞凋亡。
Objective:To investigate the effects of elemene on proliferation,cell cycle and apoptosis of human NSCLC cell line A549.Methods:Human NSCLC cell line A549cells were cultured in vitro and elemene was used.Cell Counting Kit-8assay was used to detect the proliferation of elemene.Cell cycle and apoptosis were detected by flow cytometry.Results:The inhibition rate of cell proliferation increased with the increase of the concentration of elemene.Cell cycle detection and analysis showed that after 48h and 72hours of elemene treatment,cells entering G2/M phase were significantly decreased,while cells in sub-G1phase were significantly increased.As the drug concentration increases,the apoptotic rate increases significantly.Conclusion:β-elemene can significantly inhibit the proliferation of non-small cell lung cancer A549cells,block cell cycle,inhibit cell apoptosis,and determine the optimal drug concentration and time utilization.
作者
苏春晓
白静慧
Su Chunxiao;Bai Jinghui(Liaoning University Of Traditional Chinese Medicine,Shenyang 110032,China)
出处
《亚太传统医药》
2020年第2期21-24,共4页
Asia-Pacific Traditional Medicine
