摘要
本研究建立了分散液液微萃取-高效液相色谱法测定枸杞中溴氰菊酯和氰戊菊酯农药残留的检测方法,对萃取剂与分散剂的种类、萃取剂与分散剂的体积比、p H值、离子强度、萃取时间等影响因素进行了考察。结果表明,以甲醇为分散剂、三氯甲烷为萃取剂、萃取剂与分散剂的体积比为1∶4、pH值为7、萃取时间3 min、NaCl含量为2%时对枸杞中溴氰菊酯和氰戊菊酯萃取效果最佳。氰戊菊酯和溴氰菊酯分别在0.13~26.00μg/mL(r=0.9992)和0.17~34.00μg/mL(r=0.9998)浓度范围内线性关系良好,相对标准偏差为5.25%~5.80%。
A method for the determination of deltamethrin and fenvalerate pesticide residues in Lycium barbarum was established by dispersive liquid-liquid microextraction and high performance liquid chromatography.The influencing factors such as the types of extractant and dispersant,volume ratio of extractant and dispersant,pH value,ionic strength and extraction time were investigated.The results showed that when methanol was used as dispersant,trichloromethane as extractant,the volume ratio of extractant and dispersant was 1∶4,pH value was 7,the extraction time was 3 min,and the NaCl content was 2%,it had the best extraction effect for deltamethrin and fenvalerate in Lycium barbarum.Fenvalerate and deltamethrin had a good linear relationship in the concentration range of 0.13~26.00μg/mL(r=0.9992)and 0.17~34.00μg/mL(r=0.9998),respectively,and the relative standard deviation was 5.25%~5.80%.
作者
周生葆
宋亚会
刘桂香
刘言娟
ZHOU Sheng-bao;SONG Ya-hui;LIU Gui-xiang;LIU Yan-juan(College of Pharmacy,Linyi University,Linyi Shandong 276000)
出处
《现代农业科技》
2020年第9期104-105,109,共3页
Modern Agricultural Science and Technology
基金
临沂大学博士科研启动经费(LYDX2016BS050)
2016年临沂大学大学生创新创业项目(201810452215)。
关键词
枸杞
分散液液微萃取
高效液相色谱法
农药残留
溴氰菊酯
氰戊菊酯
Lycium barbarum
dispersive liquid-liquid microextraction
high performance liquid chromatography
pesticide residue
deltamethrin
fenvalerate
